Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (3): 313-317.doi: 10.7525/j.issn.1673-5102.2011.03.011
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ZHENG Gui-Ling;LI Peng
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Abstract: Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper. The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction. A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T, including AAPT1 gene controlled by CaMV35s promoter in this experiment. Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way.
Key words: AAPT1 gene, RNAi, plant transformation vector
CLC Number:
Q291
ZHENG Gui-Ling;LI Peng. Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi[J]. Bulletin of Botanical Research, 2011, 31(3): 313-317.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2011.03.011
https://bbr.nefu.edu.cn/EN/Y2011/V31/I3/313
