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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 93-105.doi: 10.7525/j.issn.1673-5102.2020.01.014

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Transcriptome of Single and Double Tepals of Clematis patens

XIONG Yang-Yang, LUO Lin-Li, ZHAO Shi-Hao, ZHOU An-Long, WANG Jin   

  1. Institute of Landscape, Southwest Forestry University, Kunming 650224
  • Received:2019-02-18 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    State-level promotion project of forestry science and technology achievements of state forestry administration "demonstration and promotion of clematis excellent variety propagation technology"(201528)

Abstract: With the typical Clematis patens ‘Vyvyan Pennell’ which can open either single petal or double petals as the research object, we chose high throughput sequencing to splice and annotate the sequencing data of three different petal types(single petal, semi-double petal and double petal) on the same plant at the same period of C.patens ‘Vyvyan Pennell’, then selected differentially expressed genes to real-time fluorescence quantitative PCR. There were 13.8 GB raw data. A total of 3 075 differentially expressed genes(DEG) were obtained from the three transcriptome libraries by paired comparison, including 649 upregulated DEG and 605 upregulated DEG in the comparison between single and semi-double petals samples(A vs B). The comparison of semi-double flowers with double flowers(B vs C) included 1 046 up-regulated DEG and 721 down-regulated DEG. There were 1 129 upregulation DEG and 859 upregulation DEG in the comparison between single and double flowering(A vs C). There were 134 differentially expressed genes coexisting under three different perianth. By gene functional annotation, 26 genes that might be related to the double petal trait were screened out from the total DEG for cluster analysis, and 10 target genes were randomly selected for fluorescence quantitative PCR verification. PCR results showed that the expression levels of these genes were significantly different in the three floral envelope types of the same plant at the same time of C.patens ‘Vyvyan Pennell’. Finally, the key genes associated with double tepals Clematis conversion were screened out, including MADS-BOX genes PMADS1, AP3, FRUITFULL and FLC. Auxin reactive protein IAA9, auxin input vector, abscisic acid 8' hydroxylase, indoleacetic acid-induced protein ARG7, etc. This study provides basic data and theoretical basis for exploring the molecular mechanism of Clematis double flower.

Key words: Clematis patens, single and double tepals, high throughput sequencing, fluorescence quantitative PCR

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