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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 628-631.doi: 10.7525/j.issn.1673-5102.2015.04.024

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Enzyme Assisted Ethanol Extraction of Total Flavonoids from Peony Episperm

MENG Qing-Huan1;ZU Yuan-Gang1;WANG Hua2;WANG Hong-Zheng1;JIANG Tao3;WU Wei-Wei1;ZHONG Chen1;DUAN Xi-Hua1*   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040;
    2.Institute of Nature and Ecology,Heilongjiang Academy of Sciences,Harbin 150040;
    3.Wenzhou No.22 Senior Middle School,Wenzhou 325003
  • Online:2015-07-20 Published:2016-03-09
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Abstract: We optimized enzyme assisted ethanol extraction of total flavonoids from peony episperm. We studied the effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, enzymolysis time on the extraction rates of total flavonoids by singlefactor and orthogonal test. The optimal extraction conditions were after enzyming pretreatment under the pectinase content of 0.05 mg·mL-1, enzymolysis temperature of 45℃, enzymolysis pH of 4.5, enzymolysis time of 180 min, total flavonoids yield was up to 74.839 mg·g-1.Compared with the traditional ethanol extraction, the total flavonoids yield was improved significantly.

Key words: peony episperm, flavonoids, pectinase, cellulase

CLC Number: