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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 632-637.doi: 10.7525/j.issn.1673-5102.2015.04.025

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Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities

LUO Meng1,2;SONG Zhuo-Yue1,2;HU Jiao-Yang1,2;MU Fan-Song1,2*;QIAO Qi1,2;ZU Yuan-Gang1,2   

  1. 1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;
    2.The Development and Utilization Collaborative Innovation Center of Forest Economic Resources,Harbin 150040
  • Online:2015-07-20 Published:2016-03-09
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Abstract: We used ultrasonic-assisted extraction(UAE) to extract the total flavonoids from Crataegus pinnatifida leaves, and used Box-Behnken design(BBD) combined with response surface methodology(RSM) to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents(TFC). The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg·g-1. By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC50 value of 0.69 mg·mL-1. Compared with conventional maceration extraction(ME) and heat-refluxing extraction(HE) methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from C.pinnatifida leaves and its extracts were potential antioxidant ingredients.

Key words: Crataegus pinnatifida leaves, ultrasonic-assisted extraction, total flavonoids, Box-Behnken design, antioxidant activity

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