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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (3): 467-474.doi: 10.7525/j.issn.1673-5102.2018.03.020

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Flavonoids Enrichment from Elaeagnus angustifolia Fruit

ZHANG Zi-Dong, ZHANG Yuan-Yuan, MENG Yong-Bin, XU Lei, JIA Nan, XIANG Feng-Ying, LU Qi   

  1. Key Laboratory of Forest Plant Ecology of Northeast Forestry University, Ministry of Education, Harbin 150040
  • Received:2017-12-18 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    Forestry Industry Research Special Funds for Public Welfare Projects(201504701-2-4);Reserve Talent Project Funds of Heilongjiang Province of China(2017RAQXJ147)

Abstract: Based on the static screening experiments, AB-8 resin was determined to be the best resin for enriching flavonoid of Elaeagnus angustifolia from nine kinds of macro-porous resins. According to the dynamic experiment results, some enriching conditions were optimized, as follows:the sample concentration was 2.43 mg·mL-1; the flow rate was 2 BV·h-1; the ethanol concentration was 70%; the elution rate was 2 BV·h-1. The purity of total flavonoids from E. angustifolia was increased by 5.09 times, from 9.73% to 49.52%. The purity of Hypericin was increased by 5.79 times, from 0.53% to 3.07%. The results of this research would provide the theoretical basis and data support for the development and utilization of total flavonoids in E. angustifolia.

Key words: Elaeagnus angustifolia, flavonoids, enrichment

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