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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (5): 772-779.doi: 10.7525/j.issn.1673-5102.2022.05.008

• Genetic and Breeding • Previous Articles     Next Articles

Comparison of Genetic Diversity Between Wild and ex-situ Conservation Populations of Cupressus chengiana

Ermei CHANG1,2, Jianfeng LIU1,2, Yuening HUANG1,2, Hongli LI3, Bingyan SHAN4, Zeping JIANG1,5, Xiulian ZHAO1,2()   

  1. 1.Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091
    2.State Forestry Administration Key Laboratory of Tree Breeding and Cultivation,Beijing 100091
    3.Beijing Forestry University,Beijing 100083
    4.Agriculture and Rural Bureau of Yongnian District,Handan,Hebei 056011
    5.Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091
  • Received:2022-03-29 Online:2022-09-20 Published:2022-09-15
  • Contact: Xiulian ZHAO E-mail:zhaoxl@caf.ac.cn
  • About author:CHANG Ermei(1981—),female,doctor,major in biology of ancient trees.
  • Supported by:
    Horizontal project of Research Institute of Forestry Chinese Academy of Forestry(C258)


To evaluate the genetic diversity level and provide theoretical basis of ex-situ conservation populations of Cupressus chengiana, the genetic diversity, genetic structure, and gene flow of ex-situ conservation populations and wild populations of C. chengiana were studied respectively. The principal component analysis(PCA), cluster analysis, construction of molecular evolutionary tree, genetic diversity and genetic structure of three wild populations and ex-situ conservation populations based on SNP locus were obtained respectively from Genotyping-by-Sequencing technology(GBS) sequencing. The results showed that high quality clean data 118 321 514 728 bp was obtained by GBS sequencing, and 1 947 047 tags were developed. After being screened, a total of 1 259 610 of high-quality SNP sites were retained. The valley value of cross validation error rate determined that the optimal number of clusters was 1. The phylogenetic tree indicated that most of individuals ex-situ conservation populations and wild populations were clustered together. Both of combined ex-situ conservation populations and wild populations of C. chengiana had higher genetic diversity, and their value of heterozygosity(Ho), expected heterozygosity(He), Shannon information index(Shi), inbreeding coefficient(Fis), and percentage of polymorphic loci(Pic) were 0.181 5-0.272 0, 0.223 2-0.300 3, 0.331 0-0.464 9, 0.178 0-0.246 5 and 0.272 2-0.309 2, respectively. Genetic diversity of ex-situ conservation cultivated populations was relatively higher than the wild populations of C. chengiana, the value of He and Shi was 0.300 3 and 0.464 9, respectively. The wild populationsof C. chengiana of Baiwan tunnel(BW)_vs_Songgang town(SA) had the greater genetic differentiation and the gene flow(Nm) were lower than other populations(Fst=0.091,Nm=2.496). The results indicated that genetic differentiation had not occurred among these C. chengiana populations, and the gene had exchanges frequently among the ex-situ conservation populations and wild populations of C. chengianaFst<0.05, Nm>4). There was a close genetic relationship between the ex-situ conservation individuals and the wild populations individuals, and there was no obvious clustering phenomenon. Thus, transplanting endangered plants was a better method in ex-situ conservation. This paper would provide a reference for the ex-situ conservation populations of wild populations C. chengiana and provided a theoretical reference for the conservation of other tree germplasm resources.

Key words: Cupressus chengiana, wild population, ex-situ conservation population, GBS, genetic diversity, genetic structure

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