Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress

doi:10.7525/j.issn.1673-5102.2021.04.019

Abstract

Abstract:

In order to uncover the response of phenolic compounds of the Catharanthus roseus under salt stress regulated by exogenous nitric oxide（NO）， the ingredient and content of phenolic metabolites in roots， stems， leaves， flowers of the C. roseus were detected using Liquid Chromatography-mass spectrometry（LC-MS） technology after being treated with different concentrations of Sodium Nitroprusside（SNP） under salt stress respectively. The results showed that 5 kinds of C6C1 phenolic compounds including vanillic acid， p-Hydroxybenzoic acid， Syringic acid， Protocatechuic acids and Gallic acid， and 5 kinds of C6C3 phenolic compounds including trans-p-Hydroxycinnamic acid， Chlorogenic acid， Ferulic acid， Cinnamic acid and p-Coumaric acid and 8 kinds of C6C3C6（flavonoids） including Genistein， Petunidin， Naringenin， Quercetin-3-O-rhamnoside， Myricitrin， Quercetin， Kaempferol and Rutin and L- phenylalanine were identified respectively. Among them， quercetin， chlorogenic acid， protocatechuic acids were all in roots， stems， leaves and flowers. The responsive accumulation of the phenolic compounds in different tissues of C. roseus were significantly different under different concentrations of SNP treatment respectively. The small molecular phenolic acids including C6C1and C6C3 were mainly accumulated in roots and stems and C3C6C3 phenolic compounds were enriched mainly in surface leaves and flowers respectively. There were more L-phenylalanine in stem and leaf than in root and flower， and the relative content of L-phenylalanine in stem significantly increased under salt stress， but it showed a downward trend with the increase of NO concentration. Exogenous NO affected the accumulation and change of phenolic compounds in C. roseus under salt stress， and the roots and stems were more sensitive to the response. From the perspective of species and relative content， the stems and leaves were more suitable for the detection of phenolic compounds than others.

Key words: slat stress, nitric oxide, Catharanthus roseus, metabolome, phenolic compounds

CLC Number:

Q945.78

Xiao-Ju ZHAO, Yi-Ting ZHANG, Jia LIU, Yang LIU, Zhong-Hua TANG. Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress[J]. Bulletin of Botanical Research, 2021, 41(4): 633-640.

Figures/Tables 6

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Table 1

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结构类型Structure type	化合物名称Compound name	R	R1	R2	R3	R4	R5	R6
C6C1	香草酸Vanillic acid	=O	H	-OCH₃	-OH	H	/	/
	对羟基苯甲酸p-Hydroxybenzoic acid	=O	H	H	-OH	H	/	/
	丁香酸Syringic acid	=O	H	OCH₃	-OH	-OCH₃	/
	原儿茶酸Protocatechuic acid	=O	H	-OH	-OH	H	/	/
	没食子酸Gallic acid	=O	H	-OH	-OH	-OH	/	/
C6C3	对羟基肉桂酸trans-p-Hydroxycinnamic acid	-OH	H	-OH	H	H	/	/
	绿原酸Chlorogenic acid	R7	-OH	-OH	H	H	/	/
	阿魏酸cis-Ferulic acid	-OH	-OCH₃	-OH	H	H	/	/
	肉桂酸Cinnamic acid	-OH	H	H	H	H	/	/
	对香豆酸cis-p-Coumaric acid	-OH	H	-OH	H	H	/	/
C6C3C6	染料木素Genistein	H	-OH	-OH	=O	H	*	/
	木犀草苷Petunidin	H	R8	-OH	=O	R9	-OH	-OH
	柚皮素Naringenin	H	-OH	-OH	-OH	H	H	-OH
	槲皮苷Quercetin-3-O-rhamnoside	-OH	-OH	-OH	=O	R10	H	-OH
	杨梅苷Myricitrin	-OH	-OH	-OH	=O	R11	-OH	-OH
	槲皮素Quercetin	H	-OH	-OH	=O	-OH	-OH	-OH
	山柰酚Kaempferol	H	-OH	-OH	=O	-OH	H	-OH
	芦丁Rutin	H	-OH	-OH	=O	R12	-OH	-OH

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