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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 604-610.doi: 10.7525/j.issn.1673-5102.2018.04.016

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Ultrasound-Assisted Enhancement of Hair Induction Efficiency and Transgenic Hairy Root Construction of Catharanthus roseus

JIA Shan-Shan, WANG Yan-Yan, YU Fang   

  1. School of biological engineering, Dalian Polytechnic University, Dalian 116034
  • Received:2018-04-03 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    National Natural Science Foundation of China(31570303)

Abstract: Based on the induction of hairy roots of Catharanthus roseus by different strains, different inducing sites, different media and different induction methods, we tried to change the infective reagents and the new ultrasound-assisted method. By comparing the hairy root induction efficiency and hair Morphological differences in root shape, we screened the optimal method and conditions for the induction of hairy roots of C.roseus, and established transgenic hairy roots. Finally, we used C58C1 as the inoculative strain. The leaves of the aseptic seedlings were used as explant materials, 1/2 MS medium was used as the induction medium for 2-day preculture time. The co-culture buffer was used to infect for 5 s. Ultrasound-assisted induction of optimal induction method succeeded in obtaining milky white, dense, non-directional and multi-branched hairy roots, and successfully constructing the hairy roots of GUS gene, which are related to metabolic regulation pathways later. This study laid the foundation on gene function and the synthesis of secondary metabolites of the C.roseus.

Key words: Catharanthus roseus, hairy root-inducing, Agrobacterium rhizogenes, ultrasound assisted, GUS gene

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