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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 526-534.doi: 10.7525/j.issn.1673-5102.2018.04.006

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Relationship Between Phospholipase Dδ and Nitric Oxide Responding to the Seed Germination in Arabidopsis thaliana under Drought Stress

ZHANG Xu-Qiang, ZHAO Min, CHEN Lu, AN Yan-Huang, YANG Peng-Jun, Wang Xin-Xia, YANG Ning   

  1. College of Life Sciences, Northwest Normal University, Lanzhou 730070
  • Received:2018-01-29 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    National Natural Science Foundation of China(31660116)

Abstract: With Arabidopsis wild-type(WT), nitric oxide synthase(NOS) deletion mutant(noa1), nitrate reductase(NR) deletion mutant(nia1 and nia2) and PLDδ deletion mutant(pldδ) of seedlings, we studied the relationship between Arabidopsis thaliana PLDδ and nitric oxide(NO) in response to the seed germination under drought stress. NO content, PLD and NR activity and gene relative expression were significantly increased under drought stress, while pldδ and nia2 are more sensitive than other mutants to drought stress. Sodium nitroprusside(SNP), as an exogenous NO donor, can improve the seed germination of WT, nia2 and pldδ. The exogenous phosphatidic acid(PA) can promote seed germination of WT and pldδ under drought stress except nia2. Besides, in compensation experiment, PA can promote the production of NO in WT and pldδ under drought stress, but not nia2 do. Therefore, it is speculated that PLDδ/PA located at the upstream of NO signal under drought stress, and the NO produced by NR2 is the main way for PLDδ/PA to promote Arabidopsis seed germination under drought stress.

Key words: drought stress, phospholipase Dδ, nitric oxide, nitrate reductase

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