Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’

doi:10.7525/j.issn.1673-5102.2020.04.005

Abstract

Abstract: With the filaments of Paeonia ostii as explants, the effect of developmental stage of explant, basal media and PGRs on callus induction, proliferation and differentiation were investigated, and the morph-histological observation of different types of callus was performed. The translucent and white filaments from flower bud of 13-19 mm in diameter is optimal for callus induction. The optimal callus induction medium was SH containing 1 mg·L^-1 2,4-D, 2 mg·L^-1 NAA and 0.2 mg·L^-1 BA. The optimal proliferation medium was SH containing 0.5 mg·L^-1 2,4-D, 0.5 mg·L^-1 NAA and 0.25 mg·L^-1 BA, and the proliferation rate was 3.86. The filaments callus can be divided into three types according to their morphological and microstructural characteristic, the type Ⅰ callus presented typical embryogenic callus characteristics, and somatic embryogenesis was observed from this kind of callus; there were plenty of vascular tissues formed in the type Ⅱ and type Ⅲ callus, and shoot organogenesis was observed from the type Ⅱ callus, but no morphogenesis from the type Ⅲ callus. These results suggested that the filaments have important regeneration potential as a good novel explant source, which have important value in establishing in vitro regeneration system of tree peony.

Key words: Paeonia ostii ‘Feng Dan’, filament, callus

CLC Number:

S685.11

DU Yi-Ming, ZHONG Yuan, SHANG Hong-Qin, CHENG Fang-Yun. Callus Induction and Differentiation from the Filaments of Paeonia ostii ‘Feng Dan’[J]. Bulletin of Botanical Research, 2020, 40(4): 514-522.

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