Cloning and expression analysis of 5 ZFP genes from Poplus trichocarpa

doi:10.7525/j.issn.1673-5102.2020.02.011

Abstract

Abstract: ZFPs are a class of transcription factors in plants that have a loop domain. In this study, 5 ZFP genes(named PtrZFP1-5) were identified from Populus trichocarpa, and their sequence characteristics and expression patterns were analyzed to understand the stress response function. The bioinformatics analysis of PtrZFP1-5 gene was carried out, and the expressions of 5 PtrZFP genes in root, stem and leaf of Populus under NaCl, PEG₆₀₀₀ and ABA treatment were further analyzed by qRT-PCR. The number of amino acid residues encoded by PtrZFP1-5 gene was 258-338 aa, the molecular weight of the encoded protein was 27.7-37.3 kDa,and the theoretical isoelectric point was 4.87-8.61. 5 PtrZFP genes were unevenly distributed on the three chromosomes of Populus genome. The results of qRT-PCR showed that the expression patterns of 5 PtrZFP genes in roots, stems and leaves of Populus were significantly different after treatment with 0.2M NaCl, 15%(w/v) PEG6000or 100μM ABA treatment. The expressions of PtrZFP1 were all up-regulated in three kinds of stresses. The expressions of PtrZFP2 were significantly inhibited in leaves after salt, osmotic and ABA treatment. The expression changed of PtrZFP3 in roots was most obvious under drought stress. In leaves and stems, the expression level did not change significantly at most of the time points of most stress. The PtrZFP4 gene also responded to drought stress in roots and stems. The expression of PtrZFP5 gene in leaves was significantly reduced after exposure to salt and ABA stress. PtrZFP1-5 genes can respond to at least one stress treatment in one organ, but the type and mechanism of the involved stress response may be different.

Key words: ZFP, transcription factor, Populus trichocarpa, abiotic stress

CLC Number:

Q943.2

LI Ya-Bo, Lü Jia-Xin, TAN Bing, GAO Cai-Qiu. Cloning and expression analysis of 5 ZFP genes from Poplus trichocarpa[J]. Bulletin of Botanical Research, 2020, 40(2): 243-250.

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