Abstract: In order to rationally protect and utilize the important wild traditional Chinese medicine resources, we built an efficient and instability regeneration system of Lilium fargesii Franch. by embryonic callus induction. With the scales as explants, by an orthogonal experiment, we studied the effect of different kinds and contents of plant hormone on embryonic callus induction and plantlet regeneration, and established a propagation system by tissue culture. The optimal medium for inducing embryonic callus was MS+6-BA 0.5 mg·L^-1+NAA 0.5 mg·L^-1+2,4-D 0.1 mg·L^-1, the callus induction rate was 89.29%, and the generation coefficient of bulblet was 4.7. The optimal medium for regeneration of embryonic callus and inducing the bulblets was MS+6-BA 1.0 mg·L^-1+2,4-D 0.1 mg·L^-1, and its regeneration rate was 5.0/35d. The optimal rooting medium was 1/2MS+NAA 0.2 mg·L^-1, and the rooting rate was 100%. There were 90% of plantlet surviving after transplanting rooted plantlets into sand when cultured in constant temperature and humidity condition for 35 d. Our study could provide a basis on protecting the improved trait of wild resources of L.fargesii, and lay the foundation for expanding the artificial cultivation and embryoid asexual breeding.

Key words: Lilium fargesii Franch, embryonic callus, bulblet, tissue culture