Influences of Hormone Treatment and Temperature Stratification on Morphological After-ripening of Magnolia sieboldii K. Koch Seeds

doi:10.7525/j.issn.1673-5102.2015.01.007

Abstract

Abstract: We studied the main factors with different levels for morphological after-ripening of Magnolia sieboldii seeds through repeated experiments with multi factorial. When the seeds of M.sieboldii were treated by soaking in the mixed solutions with 1.30-1.40 mg·L^-1 6-BA and 2.00-2.20 mg·L^-1 2,4-D for three days, mixing the seeds and sand (1∶4), with temperature change in 100-114 d, 18±2℃ and 8h in day, 7±2℃ and 16 h in night, the incidence rate of the heart-shape embryo was 97.8%, the incidence rate of the embryo with cotyledons was 95.6%, and the germination rate of seed was 98.0%. The morphological after-ripening of seed of M.sieboldii is suitable for industrial seedlings of M.sieboldii.

Key words: Magnolia sieboldii K. Koch, seed, hormone, temperature stratification, after-ripening, influence

CLC Number:

GU Di-Zhou1；ZHUO Pan-Quan2；ZHANG Li-Fan1；WANG Qiu-Shuang1；ZHANG Xue-Shi1；ZHOU You1；ZHU Jun-Yi1. Influences of Hormone Treatment and Temperature Stratification on Morphological After-ripening of Magnolia sieboldii K. Koch Seeds[J]. Bulletin of Botanical Research, 2015, 35(1): 34-38.

References

1.张绪成，赵秉义，杨文峰.天女木兰精油的化学成份及应用［J］.吉林林业科技，1993(2)：13-15.

2.王茹萍，吴迪，高慧媛，等.天女木兰叶中甾类化合物的分离与鉴定［J］.沈阳药科大学学报，2009，26(11)：874-877.

3.陆秀君，王妮妮，李天来，等.不同浸种和催芽处理对天女木兰种子的催芽效果［J］.西北农林科技大学学报:自然科学版，2008，36(5)：135-140.

4.王妮妮，陆秀君，黄桂凤，等.不同温度条件对天女木兰种子层积催芽效果的影响［J］.西北林学院学报，2011,26(1)：82-85.

5.杜凤国，王欢，杨德冒，等.天女木兰种子形态及生物学特性［J］.北华大学学报:自然科学版，2006,7(3)：269-272.

6.姚进，黄坚钦，胡恒康，等.香榧体细胞胚发生的初步研究［J］.浙江农林大学学报,2013,30(1)：129-135.

7.冉佳鑫，王玉宇，宋丹，等.领春木体细胞胚胎发生及植株再生［J］.植物生理学报,2012,48(10):993-996.

8.尤扬，金典生.月季“黄和平”体细胞胚诱导的初步研究［J］.湖北农业科学,2012,51(10):2128-2131.

9.龚雪琴，由翠荣，曲复宁，等.朱顶红体细胞胚胎发生及植株再生研究［J］.园艺学报,2012,39(2):381-386.

10.顾地周，冯颖，顾美影，等.基于均匀设计优化手参体细胞胚胎发生及植株再生体系［J］.中草药，2010,41(6):989-993.

11.Oh E,Kim J,Park E,et al.PIL5,a phytochrome-interacting basic helix-loop-helix protein,is a key negative regulator of seed germination in Arabidopsis thaliana［J］.Plant Cell,2004,16:3045-3058.

12.Tyler L,Thomas S G,Hu J,et al.Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis［J］.Plant Physiol,2004,135:1008-1019.

13.Ryu J S,Kim J I,Kunkel T,et al.Phytochrome-specific type 5 phosphatase controls light signal flux by enhancing phytochrome stability and affinity for a signal transducer［J］.Cell,2005,120:395-406.

14.方志荣，苏智先，胡进耀.脱落酸、赤霉素和乙烯对种子休眠的萌发和调控［J］.西华师范大学学报:自然科学版,2007,28(2):127-132.

15.Yamauchi Y,Ogawa M,Kuwahara A,et al.Activation of gibberellin biosynthesis and response pathways by low temperatureduring imbibition of Arabidopsis thaliana seeds［J］.Plant Cell,2004,16:367-378.

16.陆秀君，刘月洋，陈晓旭，等.天女木兰种子后熟期间的生理生化变化［J］.北京林业大学学报，2009,31（6）：164-168.

17.刘月洋，陆秀君，李天来，等.GA₃处理对天女木兰种子解除休眠过程中碳水化合物含量的影响［J］.种子，2010，29(2)：13-17.

18.陆秀君，刘月洋，李天来，等.变温层积过程中GA₃诱导天女木兰种子贮藏物质及糖代谢关键酶活性的变化［J］.沈阳农业大学学报,2012,41(1):18-22.

19.刘月洋.天女木兰种子层积过程中物质变化及其与种子萌发的关系［D］.沈阳：沈阳农业大学,2010.

20.乌凤章，刘桂丰，姜静，等.种子萌发调控的分子机理研究进展［J］.北方园艺，2008(2):54-58.

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