Analysis adventitious shoot polarization and plantlet regeneration of Poplar- Zhonglinmeihe

doi:10.7525/j.issn.1673-5102.2006.03.016

Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (3): 329-332.doi: 10.7525/j.issn.1673-5102.2006.03.016

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Analysis adventitious shoot polarization and plantlet regeneration of Poplar- Zhonglinmeihe

ZENG You-Ling;YI Li-Juan;ZHANG Fu-Chun*

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering，College of Life Science and Technology, Xinjiang University，Urumqi 830046

Received:1900-01-01 Revised:1900-01-01 Online:2006-05-20 Published:2006-05-20
Contact: ZHANG Fu-Chun
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Abstract

Abstract: Inducing adventitious shoots were produced from different explants of Populus sp. by tissue culture on 1/2MS or MS medium with different hormone arrays which were 6BA plus NAA or IBA.The results showed that the adventitious shoot inducing rate of leafstalk was higher than leaf and stem, the adventitious shoots polarization of stem cambium was also good. The optimal medium of leafstalk was 1/2MS+6-BA0.5 mg·L^-1+ NAA0.05 mg·L^-1, adventitious shoots grew very well,inducing adventitious shoot rate was 76%,shoot number was 4.7 per leafstalk. Adventitious shoots could become whole plants by adventitious shoots multiplication,strength and radication.

Key words: Populus-Zhonglinmeihe, explant, hormone, salt concentration, adventitious shoot polarization

CLC Number:

ZENG You-Ling;YI Li-Juan;ZHANG Fu-Chun*. Analysis adventitious shoot polarization and plantlet regeneration of Poplar- Zhonglinmeihe[J]. Bulletin of Botanical Research, 2006, 26(3): 329-332.

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Analysis adventitious shoot polarization and plantlet regeneration of Poplar- Zhonglinmeihe

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