Cloning and Expression Analysis of CkGR Gene in Caragana korshinskii Kom.

doi:10.7525/j.issn.1673-5102.2016.04.005

Abstract

Abstract: A novel GR genewas isolated from Caragana korshinskii Kom. by RACE. The full-length cDNA of GR was 2122 bp, containing a 5'-UTR of 57 bp, a 3'-UTR of 415 bp, and a 1650 bp opening reading frame(ORF). The deduced protein was 550 amino acids with molecular weight 59.2 kDa and isoelectric point 8.2, named CkGR. This CkGR showed high identities with the Cicer arietinum CaGR(90.6%). The promoter of CkGR gene was isolated by chromosomal walking and 648 bp sequence was obtained by sequencing. Plant CARE analysis of this sequence showed that the peomoter contained some typical elements CAAT-box and TATA-boxand kinds of Cis-acting elements involved in defense and stress responsiveness. RT-PCR analysis revealed that CkGR was expressed in roofs, stems, and leaves with almost no tissue specificity. The transcript level of CkGR was increased in response to cold, high salt and drought stress. CkGR played an important role during cold, high salt and drought stress in Caragana korshinskii Kom..

Key words: Caragana korshinskii Kom., CkGR gene, molecular cloning, expression analysis

CLC Number:

S793.3

ZHANG Teng-Guo, ZHOU Ke, MAO Yu-Shan, NIE Ting-Ting, LI Ping, DIAO Zhi-Hong, WANG Juan. Cloning and Expression Analysis of CkGR Gene in Caragana korshinskii Kom.[J]. Bulletin of Botanical Research, 2016, 36(4): 511-519.

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