Abstract: In this research, a new expression vector of pCAMBIA1301-35S promoter-igf-1-nos, which included the gene of hIGF-1, was constructed. The gene is promoted by CaMV 35S promoter which can be expressed in tobacco in high performance. The expression vector of IGF-1 cDNA introduced by 35S promoter was transformed into LBA4404 (Agrobacterium tumefacients), and then infected tobacco through leaf-disc infection. 17 transgenic tobacco plants were gained after hygromycin selection, GUS test and PCR test of DNA. It shows IGF-1 gene can be transcribed into normal mRNA in transgenic tobacco by RT-PCR test. In this experiment, the expression of IGF-1 in tobacco lays the foundation for producing IGF-1 in tobacco and other dicotyledon bio-reactors as well.

Key words: Insulin-like growth factor I(IGF-1), tobacco, plant bio-reactor