Abstract: The genetic relationship of 17 Rosa plants is analyzed by ISSR and morphological data. NTSYS2.1 is used in clustering analysis. The results showed: 13 primers were selected to perform ISSR-PCR. A total of 479 DNA bands was obtained, of which 221 bands had polymorphism, the percentage of polymorphic bands was 0.46. All these plants could be divided into three groups by morphological data: the first group was consisted of Rosa rugosa ‘Bao Dao’, Rosa chinensis ‘Sophie’s perpetral’, Rosa chinensis ‘Comtesse dll cayla’, Rosa hybrid tea‘Sophy’s Rose’, Rosa hybrid climbingroses ‘Ten Shen Qi’, Rosa chinensis ‘Da Fu Gui’, Rose hybrid bracteata ‘Mermaid’, Rosa chinensis‘Jin Ou Fan Lv’, Rosa chinensis‘Jin Fen Lian’ and Rosa chinensis ‘Yi Ji Fen’; the second group was consisted of Rosa chinensis ‘Viridiflora’, Rosa chinensis ‘Hu Zhong Yue’ , Rosa chinensis‘Yu Shi Zhuang’ , Rosa hybrid tea ‘Yankee Dodle’, Rosa chinensis‘Si Mian Jing’ and Rosa chinensis ‘Pu Fu Hong’; the third group was consisted of Rosa gallica ‘Versicolor’. All these plants can be divided into four groups by ISSR cluster analysis. The first group was consisted of Rosa rugosa ‘Bao Dao’; the second group was consisted of Rosa chinensis ‘Viridiflora’, Rosa chinensis‘Pu Fu Hong’, Rosa chinensis‘Comtesse dll cayla’, Rose hybrid bracteata ‘Mermaid’, Rosa chinensis‘Si Mian Jing’, Rosa chinensis ‘Hu Zhong Yue’ and Rosa chinensis‘Yu Shi Zhuang’; the third group was consisted of Rosa hybrid tea ‘Sophy’s Rose’ and Rosa hybrid tea ‘Yankee Dodle’; and the fourth group was consisted of Rosa gallica ‘Versicolor’, Rosa chinensis ‘Jin Ou Fan Lv’, Rosa hybrid climbingroses ‘Ten Shen Qi’, Rosa chinensis ‘Sophie’s perpetral’, Rosa chinensis ‘Yi Ji Fen’, Rosa chinensis ‘Da Fu Gui’ and Rosa chinensis ‘Jin Fen Lian’. The results of morphologic cluster analysis and ISSR cluster analysis were similar.

Key words: Rosa L., genetic relationship, inter-mple sequence repeats(ISSR)