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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 628-632.doi: 10.7525/j.issn.1673-5102.2009.05.022

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Analysis of Genetic Relationship of Hainan Litchi Germplasm Resources by SSR Marker

YAO Qing-Rong;ZHAO Chang-Zeng;WANG Wen-Quan*   

  1. (1.Gansu Administration Institute,Lanzhou730070) (2.College of agronomy,Gansu Agricultural University,Lanzhou730070) (3.Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou571101)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: WANG Wen-Quan
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Abstract: 22 Litchi germplasm were used as materials for analyzing their genome polymorphism by SSR markers. 22 primers selected from 32 primers were used for SSR amplification. A total of 52 bands were generated, of which 49 bands were polymorphic bands. The percentage of polymorphic band (PPB) was 94.23%. According to the analysis results of POPGENE32 software, the average effective number of alleles(Ne), the Nei’s average gene diversity (H) and the average of Shannon’s genetic diversity(Hi′) index was 1.364 3, 0.296 0 and 0.417 0, respectively. The UPGAM dendrogram gotten by NTNSYS cluster analysis implied that 22 Litchi germplasm resources could be divided into 2 groups on the basis of genetic distance 0.51. The fist group included 13 cultivars. The second group included 9 cultivars.

Key words: Litchi, genetic relationship, SSR, hainan province

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