Abstract: Two genes, psaA and psbA, were amplified and sequenced for Thorea hispida(Thoreales) and blasted with other groups, then the phylogenetic trees based on two genes were generated by the methods of Neighbor-Joining, Maximum Parsimony and Bayesian. The results showed the sequences of psaA and psbA were 825 bp and 920 bp in length, respectively. A, T, C and G contents of psaA were 29.9%, 35.8%, 16.5% and 17.8% and those of psbA were 27.5%, 35.3%, 16.8% and 20.4%. There was higher content of A+T than that of G+C for two genes. It indicated that these two genes had favor in nucleotide. The phylogenetic trees generated from three methods displayed the similar topologic structures and the species of Thoreales were aggregated in one branch, separated from other groups. This result supported that Thoreales was an independent order.

Key words: psaA, psbA, Thoreales, phylogenetic relationship