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Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (5): 588-592.doi: 10.7525/j.issn.1673-5102.2007.05.018

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The Vector Construction and Prokaryotic Expression of Catharanthus roseus metallothionein(CrMT) in E.coli

ZU Yuan-Gang;FANG Si-Liang;NIE Ming-Zhu   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20
  • Contact: ZU Yuan-Gang
  • Supported by:

Abstract: A full-length sequence coding for metallothionein gene from Catharanthus roseus was cloned into the high expression vector pGEX-6P-1, and named pGEX-6P-1-CrMT. The GST-CrMT fusion protein was expressed and the expression conditions were optimized. Through the research on optimization of expression temperature, induction time and the concentration of IPTG and so on, results showed, the expression of GST-CrMT increased accompany with the induction time. The expression level of GST-CrMT fusion protein reached the highest for 24 hours cultured at 22℃ and for 240 min cultured at 37℃, 0.8 mmol·L-1 IPTG can effectively induced the expression of GST-CrMT in prokaryotic expression system.

Key words: Catharanthus roseus, metallothionein(MT), vector construction, prokaryotic expression

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