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植物研究 ›› 2007, Vol. 27 ›› Issue (3): 376-379.doi: 10.7525/j.issn.1673-5102.2007.03.024

• 论文 • 上一篇    下一篇

HPLC-ELSD法在刺五加药材糖类成分分析中的应用

郑春英1;平文祥1;刘松梅1;祖元刚2   

  1. 1.黑龙江大学生命科学学院,微生物黑龙江省高校重点实验室,哈尔滨 1500802.东北林业大学森林植物生态学教育部实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20
  • 通讯作者: 郑春英
  • 基金资助:
     

Analysis of Sugars in Acanthopanax senticosus (Rupr.et Maxim.) by HPLC with ELSD Detection

ZHENG Chun-Ying;PING Wen-Xiang;LIU Song-Mei;ZU Yuan-Gang   

  1. 1.Key Laboratory of Microbiology,Life Science College,Heilongjiang University,Harbin 150080 2.Key Laboratory of Forest Plant Ecology, Ministry of Education,Northeast Forest University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20
  • Contact: ZHENG Chun-Ying
  • Supported by:
     

摘要: 建立一种高效、快速的高效液相色谱—蒸发光散射检测法(HPLC-ELSD)分析刺五加药材中糖类成分—葡萄糖及蔗糖组分。采用CHROMATOREX NH2柱为固定相;以乙腈:水为84.5:15.5(V/V)为流动相,流速1.0 mL·min-1;漂移管温度为100℃;氮气流速为2.5 L·min-1。在上述色谱条件下,葡萄糖和蔗糖的线性范围分别为0.023 2~0.116 0 mg·mL-1及0.099 6~0.496 5 mg·mL-1;且该二种糖的检测限分别(信/噪=3)为3.87 ng和3.31 ng。本方法无须进行糖衍生化而直接用于刺五加药材中糖类成分的分离分析。

关键词: 刺五加, HPLC-ELSD, 葡萄糖, 蔗糖

Abstract: A simple and rapid HPLC coupled with Evaporative Light Scattering Detection (ELSD) method was developed for the determination of glucose and sucrose in Acanthopanax senticosus (Rupr. et Maxim.). The HPLC separation was performed on a CHROMATOREX NH2 column using acetonitrile-water (84.5:15.5, v/v) as mobile phase at a flow rate of 1 mL·min-1. The drift tube temperature of ELSD was set at 100℃, and with the nitrogen flow-rate of 2.5 L·min-1. The linear ranges were from 0.023 2 to 0.116 0 mg·mL-1 for glucose, and from 0.099 6 to 0.496 5 mg·mL-1 for sucrose. All regression coefficients were more than 0.999 0. The detection limits (Signal/Noise=3) obtained were 3.87 ng and 3.31 ng for glucose and sucrose, respectively. The method was first used for separation and determination of sugars in Traditional Chinese herb Acanthopanax senticosus (Rupr. et Maxim.) without prior derivatization, and the results were satisfactory.

Key words: Acanthopanax senticosus (Rupr. et Maxim.), HPLC-ELSD, glucose, sucrose

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