欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2013, Vol. 33 ›› Issue (4): 490-493.doi: 10.7525/j.issn.1673-5102.2013.04.017

• 论文 • 上一篇    下一篇

高效液相色谱法同时测定楠竹叶提取物中的4种黄酮

张学科;马春慧;杨磊*;孟庆焕;王洪政;祖元刚   

  1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-07-20 发布日期:2013-07-20
  • 通讯作者: 杨磊
  • 基金资助:
     

Simultaneous Determination of 4 Flavonoids in Extracts of Phyllostachys pubescens Leaves by HPLC

ZHANG Xue-Ke;MA Chun-Hui;YANG Lei*;MENG Qing-Huan;WANG Hong-Zheng;ZU Yuan-Gang   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-07-20 Published:2013-07-20
  • Contact: YANG Lei
  • Supported by:
     

摘要: 采用高效液相色谱法同时测定楠竹叶提取物中的4种主要黄酮成分:荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷、牡荆苷-2′-O-鼠李糖苷。通过实验,得到了同时测定4种主要黄酮成分的最佳条件。采用Kromasil-C18(5 μm,4.6 mm×250 mm,Akzol Nobel,Sweden)进行测定,最佳检测条件为:以四氢呋喃∶乙腈∶甲醇∶0.5%醋酸水溶液=10.3∶1∶0.7∶88为色谱流动相等度洗脱;检测波长360 nm;柱温25℃,流速:1.0 mL·min-1。荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷和牡荆苷-2′-O-鼠李糖苷的平均回收率分别为98.5%、98.1%、99.1%和98.7%,相对标准偏差分别为0.8%、0.7%、0.8%和0.6%(n=5)。该方法分离效率高、分析速度快、检测灵敏度高、重复性好,可用于楠竹叶成分的检测分析。

关键词: 楠竹叶提取物, 荭草苷, 牡荆苷, 牡荆苷-4&prime, -O-葡萄糖苷, 牡荆苷-2&prime, -O-鼠李糖苷, 高效液相色谱

Abstract: In this study, 4 flavonoids in Phyllostachys pubescens extracts i.e. orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were simultaneously detected. The HPLC detected conditions were as follows: Kromasil-C18 column (5 μm, 4.6 mm×250 mm, Akzol Nobel, Sweden), mobile phase was tetrahydrofuran∶acetonitrile∶methanol∶0.5% aqueous acetic acid (10.3∶1∶0.7∶88), isocratic elution, the detection wavelength was 360 nm, column temperature was 25℃, and 1.0 mL·min-1 flow velocity. The average recoveries of orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were 98.5%, 98.1%, 99.1%, and 98.7%, respectively. The relative standard deviations were 0.8%, 0.7%, 0.8% and 0.6%(n=5), respectively. This HPLC method has been proved to be a method with high efficiency, rapid analysis, high sensitivity and good reproducibility for detection of flavonoids in P.pubescens extracts.

Key words: Phyllostachys pubescens extracts, orientin, vitexin, vitexin-4&prime, -O-glucoside, vitexin-2&prime, -O-rhamnose glycoside, HPLC

中图分类号: