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植物研究 ›› 2007, Vol. 27 ›› Issue (4): 469-472.doi: 10.7525/j.issn.1673-5102.2007.04.019

• 论文 • 上一篇    下一篇

短小芽孢杆菌对盐藻SZ-05的生物量和β-胡萝卜素产量的影响

杜彩华1,2;郑维发2*;赵艳霞1,2;魏江春1;储成才3   

  1. 1.河北大学生命科学学院,保定 071002 2.江苏省药用植物生物技术重点实验室,徐州 221116 3.中国科学院遗传与发育生物学研究所,北京 100101
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-20 发布日期:2007-07-20
  • 通讯作者: 郑维发
  • 基金资助:
     

Effects of Bacillus pumilus on Biomass and β-carotene Accumulation in Dunaliella salina SZ-05

DU Cai-Hua;ZHENG Wei-Fa*;ZHAO Yan-Xia;WEI Jiang-Chun;CHU Cheng-Cai   

  1. 1.School of Life Sciences,Hebei University,Baoding 071002 2.Key laboratory for Biotechnology on Medicinal Plants of Jiangsu Province,Xuzhou Normal University,Xuzhou 221116 3.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20
  • Contact: ZHENG Wei-Fa
  • Supported by:
     

摘要: 研究了短小芽孢杆菌(Bacillus pumilus)对盐藻空间诱变株系SZ-05(Dunaliella salina SZ-05)的生物量及β-胡萝卜素积累的影响。结果表明,短小芽孢杆菌显著提高了盐藻SZ-05的生物量和β-胡萝卜素的产量,明显降低了培养体系中的溶解氧和胞外多糖的含量。溶解氧的减少,使得藻细胞的光呼吸作用下降,光合作用速率提高,使藻细胞生物量增加。胞外多糖具有抗氧化作用,胞外多糖的减少可能进一步增加了β-胡萝卜素的合成,从而使β-胡萝卜素在胁迫条件下大幅度增加。

关键词: 短小芽孢杆菌, 盐藻SZ-05, 生物量, &beta, -胡萝卜素积累, 胞外多糖, 溶解氧

Abstract: Bacillus pumilus was investigated for its effects on the accumulation of biomass and β-carotene in the culture of Dunaliella salina SZ-05. The results showed that the presence of B. pumilus significantly increased the growth rate and the accumulation of biomass and β-carotene in the culture of D. salina SZ-05, and reduced the dissolved oxygen and exopolysaccharides. The reduction of dissolved oxygen resulted in a decreased photorespiration in alga cells and hence an increased photosynthesis, and consequently an enhanced growth rate and biomass accumulation. Exopolysaccharides were found to be the active agents to quench free radicals. In stress conditions, the reduction of exopolysaccharides might triggered an enhanced biosynthesis of β-carotene, leading to an increased accumulation of β-carotene.

Key words: Bacillus pumilus, Dunaliella salina SZ-05, biomass, &beta, -carotene accumulation, exopolysaccharides (EPS), dissolved oxygen

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