组成型过表达PagPYL4基因对84K杨耐旱性与生长的影响

doi:10.7525/j.issn.1673-5102.2025.05.007

摘要/Abstract

摘要：

在气候变化和耕地资源日益紧张背景下，提升林木抗旱性成为重要育种目标。然而，抗旱性改良不应以牺牲生物量积累为代价。该研究在84K杨（Populus alba×P. glandulosa ‘84K’）中构建了组成型过表达PagPYL4基因株系，系统分析其在气孔调控、光合效率、生长性状及干旱响应方面的综合表现。结果表明，在外源ABA影响下，过表达PagPYL4基因使植株气孔开度变化显著。短期干旱处理下，相较于野生型，过表达PagPYL4基因能够减少水分散失，提升耐旱性；但抑制气孔导度和光合速率，造成株高与地径增长速率显著下降。该研究揭示了在林木抗逆改良中，仅依赖组成型表达难以实现理想育种目标，应探索更精准的表达调控策略以实现抗性与生长的协同优化。

关键词: 84K杨, PagPYL4, 耐旱, 生长, 气孔, 光合作用

Abstract:

In the context of climate change intensification and increasing limitation of arable land， improving drought tolerance in forest trees has emerged as a critical breeding goal. However， achieving enhanced stress resistance without compromising growth and biomass production remains a major challenge. In this study， transgenic 84K poplar （Populus alba×P. glandulosa ‘84K’） lines constitutively overexpressing PagPYL4 gene， an abscisic acid（ABA） receptor gene， were developed， and their physiological and growth responses were evaluated under drought stress. The results showed that under the influence of exogenous ABA， the overexpression of PagPYL4 gene significantly altered stomatal aperture in plants. Under short-term drought treatment， compared with the wild type， the overexpression of PagPYL4 gene reduced water loss and improved drought tolerance； however， it also inhibited stomatal conductance and photosynthetic rate， leading to a significant decrease in growth rate of plant height and ground diameter. Our findings demonstrated that while PagPYL4 overexpression effectively enhanced drought resistance， its indiscriminate activation can impose substantial growth penalties. This highlighted the importance of spatially and temporally regulated gene expression strategies to balance stress resilience and biomass productivity in future tree breeding programs.

Key words: Populus alba×P. glandulosa ‘84K’, PagPYL4, drought tolerance, growth, stomata, photosynthesis

中图分类号:

Q945

刘上, 王锦华, 哈斯, 刘畅. 组成型过表达PagPYL4基因对84K杨耐旱性与生长的影响[J]. 植物研究, 2025, 45(5): 722-730.

Shang LIU, Jinhua WANG, Hasi YU, Chang LIU. Effects of Constitutive Overexpression of PagPYL4 Gene on Drought Tolerance and Growth of 84K poplar[J]. Bulletin of Botanical Research, 2025, 45(5): 722-730.

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引物名称 Primer name	序列（5′→3′） Sequence（5′→3′）	应用 Application
84KPYL4-F	CAGGTCGACTCTAGAGGATCCATGCCTGCTAATCCTCCGAG	PCR/Identification
84KPYL4-R	GGGAAATTCGAGCTCGGTACCTCACGATGATGATTTATTATTGCGTC	PCR
Semi-PagPYL4-F	CGGTGGTCTCCTCTACCACGC	Semi-quantitative PCR
Semi-PagPYL4-R	GCTCATCGTCGAGGATCTCGAGG	Semi-quantitative PCR
UBQ7-F	CCTAACTGGCAAGACCATCAC	Semi-quantitative PCR
UBQ7-R	AGCCTCAGAACCAGATGCAGT	Semi-quantitative PCR
NOSR	CATCGCAAGACCGGCAACAG	Identification