拟南芥根毛功能基因AtGDPD-Like3关键氨基酸位点鉴定

doi:10.7525/j.issn.1673-5102.2020.01.012

植物研究 ›› 2020, Vol. 40 ›› Issue (1): 79-84.doi: 10.7525/j.issn.1673-5102.2020.01.012

拟南芥根毛功能基因AtGDPD-Like3关键氨基酸位点鉴定

王爽, 程玉祥, 夏德安

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2019-04-24 出版日期:2020-01-05 发布日期:2020-02-21
通讯作者: 夏德安 E-mail:xiadean@126.com
作者简介:王爽(1993-),女,硕士研究生,主要从事林木次生生长研究。
基金资助:
国家自然科学基金（31570580）

Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs

WANG Shuang, CHENG Yu-Xiang, XIA De-An

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2019-04-24 Online:2020-01-05 Published:2020-02-21
Supported by:
Natural Scientific Foundation of China(31570580)

摘要/Abstract

摘要： AtGDPD-Like3是编码甘油磷酸二酯磷酸二酯酶（GDPD）类似基因，拟南芥该家族基因AtGDPD-Like3突变体shv3存在严重的根毛发育缺陷。为了鉴定AtGDPD-Like3关键氨基酸位点，我们构建S⁵³⁸A、V⁵⁵⁶A和D⁶²⁸A单点突变AtGDPD-Like3，分别转化atgdpdl3突变体并观察其恢复根毛缺陷程度。结果显示V⁵⁵⁶A、D⁶²⁸A位点突变AtGDPD-Like3完全恢复atgdpdl3根毛生长缺陷表型，但S⁵³⁸A突变AtGDPD-Like3只是部分恢复根毛缺陷。这些结果表明Ser⁵³⁸是AtGDPD-Like3较为关键的氨基酸位点，突变影响其蛋白质功能行使，同时暗示AtGDPD-Like3还存在其它的关键氨基酸位点。此研究结果为进一步探究AtGDPD-Like3蛋白功能行使的作用机制奠定了基础。

关键词: 拟南芥, AtGDPD-Like3, 关键氨基酸位点, 点突变

Abstract: AtGDPD-Like3 encode glycerophosphodiester phosphodiesterase-like(GDPD) proteins and shv3, its family gene AtGDPD-Like3 mutant, has serious root hair defects. To identify key amino acid sites in AtGDPD-Like3, we constructed S⁵³⁸A, V⁵⁵⁶A and D⁶²⁸A single site mutation of AtGDPD-Like3, which were expressed in atgdpdl3 mutants, respectively. The results showed that AtGDPD-Like3 with V⁵⁵⁶A or D⁶²⁸A mutation could completely restore root hairs growth defects of atgdpdl3, whereas, AtGDPD-Like3 with S⁵³⁸A mutation partially restored root hairs growth defects. These indicate that Ser⁵³⁸ is the key amino acid site in AtGDPD-Like3, which affects function of its protein. Meanwhile, it implies other key amino acid sites in AtGDPD-Like3. The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.

Key words: Arabidopsis thaliana, AtGDPD-Like3, key amino acid locus, point mutation

中图分类号:

Q945.12

王爽, 程玉祥, 夏德安. 拟南芥根毛功能基因AtGDPD-Like3关键氨基酸位点鉴定[J]. 植物研究, 2020, 40(1): 79-84.

WANG Shuang, CHENG Yu-Xiang, XIA De-An. Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs[J]. Bulletin of Botanical Research, 2020, 40(1): 79-84.

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拟南芥根毛功能基因AtGDPD-Like3关键氨基酸位点鉴定

Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs

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