樟子松乙醇提取物体外抗氧化活性研究

doi:10.7525/j.issn.1673-5102.2019.03.016

植物研究 ›› 2019, Vol. 39 ›› Issue (3): 458-465.doi: 10.7525/j.issn.1673-5102.2019.03.016

樟子松乙醇提取物体外抗氧化活性研究

张力凡¹, 薛煜¹, 王岩², 杨光鑫³, 杨光¹, 邸雪颖¹

1. 东北林业大学林学院, 哈尔滨 150040;
2. 黑龙江省黑河市逊克县道干林场, 黑河 164499;
3. 国家林业局大兴安岭林业调查规划设计院, 加格达奇 165000

收稿日期:2019-03-13 出版日期:2019-05-05 发布日期:2019-05-11
通讯作者: 邸雪颖 E-mail:dixueying@126.com
作者简介:张力凡(1994-),女,硕士研究生,主要从事森林防火方面的研究。
基金资助:
北京市重点实验室开放基金项目（BJFUKF201818）

External Antioxidant Activity of Ethanol Extract from Pinus sylvestris var. mongolica

ZHANG Li-Fan¹, XUE Yu¹, WANG Yan², YANG Guang-Xin³, YANG Guang¹, DI Xue-Ying¹

1. School of forestry, Northeast Forestry University, Harbin 150040;
2. Forest Farm, Xunke County Road, Heihe 164499;
3. National Forestry Bureau Daxing'an Mountains Forestry Survey Planning and Design Institute, Gagdage 165000

Received:2019-03-13 Online:2019-05-05 Published:2019-05-11
Supported by:
The open fund project of Beijing key laboratory(BJFUKF201818)

摘要/Abstract

摘要： 测定了樟子松球果、树皮、树叶、木质部4个部位提取物的总抗氧化能力、总还原力、二苯代苦味酰基（DPPH·）清除能力、羟基自由基（·OH）清除能力、超氧阴离子（O^·₂）清除能力5个指标，并以抗坏血酸（Vc）为阳性对照评价从樟子松4个部位提取的松多酚体外抗氧化活性。结果表明：（1）从樟子松树皮中提取的樟子松多酚表现出较强的抗氧化能力，在较高浓度下与抗氧化剂（Vc）相差较小；从樟子松球果中提取的樟子松多酚也表现出较强的还原力，甚至超过阳性对照抗氧化剂Vc；（2）从樟子松树皮中提取的樟子松多酚在二苯代苦味酰基（DPPH·）清除能力、羟基自由基（·OH）清除能力上均为三者之中最高的，但都不超过抗氧化剂（Vc）的抗氧化能；（3）超氧阴离子（O^·₂）清除能力。三者的清除率均为负值，说明该方法测定超氧阴离子（O^·₂）清除率存在干扰因素，无法比较三者在这一指标上的能力大小。上述结果表明樟子松乙醇提取物具有较强的抗氧化能力，可作为天然的抗氧化剂。

关键词: 樟子松, 多酚, 乙醇提取物, 抗氧化活性

Abstract: The ethanol extractantioxidant activity of cone, bark, leaf, xylem four parts of Pinus sylvestris var. mongolica was compared to provide theoretical basis for further exploitation and utilization of pine resources. P.sylvestris var. mongolica bark, leaves, cones, xylem four parts as raw material, the content of polyphenols and flavonoids in the ethanol extract of camphor was determined. Determination of the P.sylvestris var. mongolica ethanol extracts of polyphenols, flavonoids, and ascorbic acid(VC) is applied to make positive control evaluation of the extracts from different parts of total antioxidant capacity, total reducing power, diphenyl bitter acyl(DPPH·) cleaning ability, hydroxyl free radical(·OH) clear, super oxygen anion(O₂^·) remove the five indicators, evaluation of pine ethanol extractpolyphenols ofmongolica four parts in vitro antioxidant activity, draw standard curve. The results showed that:(1)mongolica polyphenols extracted from P.sylvestris var. mongolica bark showed strong antioxidant capacity, in relatively high concentrations and antioxidants(Vc) is smaller; The camphor pine polyphenols, extracted from camphor cones, also exhibit stronger restorative powers, even more than antioxidants(Vc); (2)P.sylvestris var. mongolica polyphenols extracted from which bark in diphenyl bitter acyl(DPPH·) cleaning ability, hydroxyl free radical(·OH) removal ability are the highest of the three, but no more than antioxidants(Vc) antioxidant capacity; (3)super oxygen anion(O₂^·) cleaning ability index, the clearance of all negative, shows that the method for determining the ultra oxygen anion(O₂^·) the clearance interference factors are unable to compare three size on this metric capability. Experimental results show that the polyphenols have strong antioxidant ability, which can be used as a natural antioxidant.

Key words: Pinus sylvestris var. mongolica, polyphenol, ethanol extract, antioxidation activity

中图分类号:

S791.253

张力凡, 薛煜, 王岩, 杨光鑫, 杨光, 邸雪颖. 樟子松乙醇提取物体外抗氧化活性研究[J]. 植物研究, 2019, 39(3): 458-465.

ZHANG Li-Fan, XUE Yu, WANG Yan, YANG Guang-Xin, YANG Guang, DI Xue-Ying. External Antioxidant Activity of Ethanol Extract from Pinus sylvestris var. mongolica[J]. Bulletin of Botanical Research, 2019, 39(3): 458-465.

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樟子松乙醇提取物体外抗氧化活性研究

External Antioxidant Activity of Ethanol Extract from Pinus sylvestris var. mongolica

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