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植物研究 ›› 2017, Vol. 37 ›› Issue (4): 621-627.doi: 10.7525/j.issn.1673-5102.2017.04.019

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雪松松针挥发油化学成分及抗氧化活性研究

胡文杰1, 罗辉2, 邹林海1, 李冠喜3   

  1. 1. 井冈山大学生命科学学院, 吉安 343009;
    2. 井冈山大学医学院, 吉安 343000;
    3. 曲阜师范大学生命科学学院, 曲阜 273165
  • 收稿日期:2017-02-21 出版日期:2017-07-15 发布日期:2017-07-22
  • 通讯作者: 李冠喜,E-mail:1040411985@qq.com E-mail:1040411985@qq.com
  • 作者简介:胡文杰(1974-),男,博士,主要从事天然产物化学研究。
  • 基金资助:
    江苏省自然基金(BK20151278);江苏省苏北科技项目(BN2014029)

Chemical Components and Antioxidant Activity of Volatile Oil from Pine Needles of Cedrus deodara

HU Wen-Jie1, LUO Hui2, ZOU Lin-Hai1, LI Guan-Xi3   

  1. 1. College of Life Sciences, Jinggangshan University, Ji'an 343009;
    2. School of Medicine, Jinggangshan University, Ji'an 343000;
    3. School of Life Sciences, Qufu Normal University, Qufu 273165
  • Received:2017-02-21 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    The Natural Science Foundation of Jiangsu Province(BK20151278);Science and technology projects of northern jiangsu in Jiangsu province(BN2014029)

摘要: 采用水蒸气蒸馏法提取雪松松针挥发油,利用气相色谱-质谱联用技术分析其化学成分。使用维生素C为阳性对照,以DPPH自由基、ABTS自由基和羟基自由基清除作用为指标评价挥发油的抗氧化活性。从雪松松针挥发油中鉴定了37种化学成分,占挥发油总量的95.10%,以烃类和醇类为主;挥发油对DPPH自由基、ABTS自由基和羟基自由基有明显的清除作用,其IC50分别为0.30、0.22及0.96 μg·mL-1,且呈现一定的量效关系。可见,雪松松针挥发油中含有多种有应用价值的化学成分,具有显著的抗氧化活性。

关键词: 雪松, 挥发油, 化学成分, 气相色谱-质谱联用仪, 抗氧化活性

Abstract: The volatile oil from pine needles of Cedrus deodara was extracted by water-steam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·, ABTS· and ·OH scavenging capacity with VC as the positive control. Thirty-seven components, accounting for 95.10% of total components, were identified. Olefin hydrocarbon and alcohols were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·, ABTS· and ·OH in a concentration fashion. When the scavenging rate against DPPH·, ABTS· and ·OH of volatile oil was 50%, the contents of volatile were 0.30, 0.22 and 0.96 μg·mL-1, respectively. The volatile oil from pine needles of C.deodara contains a variety of chemical composition of application value with a certain antioxidant activity.

Key words: Cedrus deodara, volatile oil, chemical components, GC-MS, antioxidant activity

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