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植物研究 ›› 2021, Vol. 41 ›› Issue (4): 626-632.doi: 10.7525/j.issn.1673-5102.2021.04.018

• 研究报告 • 上一篇    

大孔树脂纯化岩高兰多酚的工艺研究

高媛1, 邢文馨2, 王慧梅1()   

  1. 1.东北林业大学,森林植物生态学教育部重点实验室,化学化工与资源利用学院,哈尔滨 150040
    2.东北林业大学生命科学学院,哈尔滨 150040
  • 收稿日期:2020-04-10 出版日期:2021-07-20 发布日期:2021-03-24
  • 通讯作者: 王慧梅 E-mail:whm0709@163.com
  • 作者简介:高媛(1995—),女,硕士研究生,主要从事药用植物活性成分研究。
  • 基金资助:
    中央高校基础研究项目(2572017EA03)

Macroporous Resin Purifying Polyphenols from Empetrum nigrum Aerial Parts

Yuan GAO1, Wen-Xin XING2, Hui-Mei WANG1()   

  1. 1.Key Laboratory of Forest Plant Ecology,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
    2.College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2020-04-10 Online:2021-07-20 Published:2021-03-24
  • Contact: Hui-Mei WANG E-mail:whm0709@163.com
  • About author:GAO Yuan(1995—),female,master,mainly engaged in physiology of medicinal plants.
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572017EA03)

摘要:

为获得大孔树脂纯化岩高兰多酚的最佳工艺,以岩高兰的地上部分为原料,通过考察6种不同类型树脂(HPD-100、X-5、AB-8、D101、HPD-600、NKA-II)的含水率、吸附率和解吸率的大小,筛选出一种最适合纯化岩高兰多酚的树脂。在此基础上,选择对纯化工艺影响较大的4种因素(上样浓度、乙醇浓度、洗脱流速、洗脱体积),进行响应面法分析得到最佳工艺。结果表明:HPD-600型大孔树脂对岩高兰多酚的纯化效果最佳,其最优工艺参数为:上样浓度0.84 mg·mL-1;乙醇浓度62.15%;洗脱流速0.67 mL·min-1;洗脱体积2.71 BV。该条件下,岩高兰多酚的提取率为229.18 mg·g-1,岩高兰多酚的纯度由8.11%提高到22.56%,回收率为67.78%。本研究为岩高兰多酚的纯化工艺提供了新的技术路线,也可为岩高兰提取物的研究和应用提供参考。

关键词: 岩高兰, 多酚, 大孔树脂, 响应面法, 纯化工艺

Abstract:

In order to optimize the process of polyphenols by macroporous resin from Empetrum nigrum aerial parts, moisture content, adsorption rate and desorption rate of six resins(HPD-100, X-5, AB-8, D101, HPD-600 and NKA-Ⅱ) were investigated, and the resin with the best effect was selected. Four factors(sample concentration, ethanol concentration, elution flow rate and elution volume) that have a great influence on the purification process were selected, and the response surface method was used to analyze the optimal process. HPD-600 macroporous resin had the best purification effect on polyphenols yield. The optimal extraction conditions were as follows: sample concentration was 0.84 mg·mL-1, ethanol concentration was 62.15%, elution flow rate was 0.67 mL·min-1, and elution volume was 2.71 BV. Under these conditions, the yield of polyphenols was 229.18 mg·g-1, the purity of polyphenols was increased from 8.11% to 22.56%, and the recovery rate was 67.78%.

Key words: Empetrum nigrum, polyphenols, macroporous resin, response surface method, purification process

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