不同天然居群小檗种子萌发障碍因子研究

doi:10.7525/j.issn.1673-5102.2018.06.013

植物研究 ›› 2018, Vol. 38 ›› Issue (6): 894-901.doi: 10.7525/j.issn.1673-5102.2018.06.013

不同天然居群小檗种子萌发障碍因子研究

李林瑜¹, 方紫妍¹, 艾克拜尔·毛拉¹, 周龙¹, 陆彪²

1. 新疆农业大学林学与园艺学院, 乌鲁木齐 830052;
2. 新疆特克斯县林业局, 特克斯县 835500

收稿日期:2018-05-16 出版日期:2018-11-15 发布日期:2018-11-01
通讯作者: 周龙 E-mail:zhoulong2004@126.com
作者简介:李林瑜(1990-),女,硕士研究生,主要从事植物资源保护方面的研究。
基金资助:
国家科技部重点研发计划（2016YFC0501504）；新疆农业大学校级研究生科研创新项目（XJAUGRI2017035）；新疆农业大学2612创新团队；新疆维吾尔自治区园艺学重点学科

Germination Obstacle Factors of Berberis Seeds of Different Natural Populations

LI Lin-Yu¹, FANG Zi-Yan¹, Maola Aikebaier¹, ZHOU Long¹, LU Biao²

1. College of Forestry and Horticulture, Xinjiang Agricultural University, Wulumuqi 830052;
2. Xinjiang Tekesi County Forestry Bureau, Tekesi 835500

Received:2018-05-16 Online:2018-11-15 Published:2018-11-01
Supported by:
Key R&D plan of the State Ministry of science and technology(2016YFC0501504);Research and innovation projects of graduate students at Xinjiang Agricultural University(XJAUGRI2017035);2612 innovation team of Xinjiang Agricultural University;Key disciplines of horticulture in Xinjiang Autonomous Region

摘要/Abstract

摘要： 以西天山野果林霍城居群、新源居群和特克斯居群黑果小檗以及特克斯居群红果小檗种子为试验材料，研究了4组野生小檗种子生物学特性、吸水特性以及去种皮、低温层积和不同浓度GA₃处理对小檗种子休眠与萌发特性的影响，结果表明：（1）4组野生小檗种子的吸水率均表现出逐渐增加的趋势，黑果小檗种子与红果小檗种子吸水率差异不明显，其种皮透水透气性相似；（2）霍城居群、新源居群和特克斯居群黑果小檗种子的种皮对萌发存在强烈的抑制作用；而新源居群红果小檗种子的种皮抑制作用不明显；（3）4℃低温层积处理对4组野生小檗种子萌发影响很大，随层积时间的增加小檗种子发芽率均逐渐提高，3组黑果小檗种子层积90 d时，休眠基本被解除；红果小檗种子层积30 d时，休眠基本被解除；（4）浓度为200 mg·L^-1的GA₃溶液处理可显著提高4组小檗种子发芽率，浓度过高或过低均会对小檗种子萌发起到抑制作用。层积60 d时霍城居群、新源居群和特克斯居群黑果小檗去种皮种子用200 mg·L^-1的GA₃溶液处理2 h后，发芽率分别为85.00%、83.33%和86.67%；红果小檗层积15 d时去种皮种子用浓度为200 mg·L^-1的GA₃溶液处理后，发芽率可达86.67%。研究结果将为今后野生小檗的种质资源保护，利用及可持续发展提供技术支持和科学依据。

关键词: 小檗, 天然居群, 种子萌发, 低温层积, 赤霉素

Abstract: The Black-fruit Berberis seeds of Huocheng population, Xinyuan population, Tekesi population and the Red-fruit Berberis of Tekesi population were used as experimental materials to study their biological characteristics, water absorption characteristics and the effects of seed coat removal, low temperature stratification and different concentrations of GA₃ treatment on the dormancy and germination characteristics.The results showed that:(1) The water absorption rate of four groups of wild Berberis seeds showed a trend of increasing gradually.There was no significant difference in water absorption between Black-fruit Berberis seeds and Red-fruit Berberis seeds, and the water permeability of their seed coat was similar; (2) The seed coats of Black-fruit Berberis seeds of Huocheng population, Xinyuan population and Tekesi population had a strong inhibitory effect on germination.However the seed coat of Red-fruit Berberis seeds in Tekesi population had no obvious inhibition on germination; (3) Low temperature stratification had a great effect on the germination of wild Berberis seeds in 4 groups, and the germination rate of Berberis seeds increased gradually with the increase of stratification time.The dormancy of Black-fruit Berberis seeds was basically relieved after 90 days of stratification;the dormancy of Red-fruit Berberis seeds was basically relieved after 30 days of stratification; (4) GA₃ solution with 200 mg·L^-1 concentration could significantly increase the germination rate of the four groups Berberis seeds, and too high or too low concentration could inhibit the germination of Berberis seeds. After 60 days of layering,the germination rates of Black-fruit Berberis seeds without seed coat of Huocheng population, Xinyuan population and Tekesi population were 85.00%, 83.33%, and 86.67% respectively after treated with GA₃ solution of 200 mg·L^-1 for 2 hours;When the Red-fruit Berberis seeds were layered for 15 days,the germination rates of Red-fruit Berberis seeds without seed coat of Tekesi population were 86.67% after treated with GA₃ solution of 200 mg·L^-1 for 2 hours.The results will provide technical support and scientific basis for the introduction and breeding of wild Berberis.

Key words: Berberis, natural populations, seeds germination, low temperature stratification, GA₃

中图分类号:

Q949.746.8

李林瑜, 方紫妍, 艾克拜尔·毛拉, 周龙, 陆彪. 不同天然居群小檗种子萌发障碍因子研究[J]. 植物研究, 2018, 38(6): 894-901.

LI Lin-Yu, FANG Zi-Yan, Maola Aikebaier, ZHOU Long, LU Biao. Germination Obstacle Factors of Berberis Seeds of Different Natural Populations[J]. Bulletin of Botanical Research, 2018, 38(6): 894-901.

参考文献

1. 任珺,余方可,陶玲. 荒漠植物种子逆境萌发研究进展[J]. 植物研究,2011,31(1):121-128.Ren J,Yu F K,Tao L. Research advances on the germination of desert plants under stress[J]. Bulletin of Botanical Research,2011,31(1):121-128.
2. Finkelstein R,Reeves W,Ariizumi T,et al. Molecular aspects of seed dormancy[J]. Annual Review of Plant Biology,2008,59:387-415.
3. Schopmeyer C S. Seeds of woody plants in the United States[M]. Washington:USDA,Forest Service,1974.
4. Wang B S P,Berjak P. Beneficial effects of moist chilling on the seeds of black spruce(Picea mariana (Mill.) B. S. P.)[J]. Annals of Botany,2000,86(1):29-36.
5. 潘睿,孙卫邦. 极度濒危植物大果木莲种子的休眠与萌发[J]. 植物生理学通讯,2009,45(11):1089-1092.Pan R,Sun W B. Seed dormancy and germination of the critically endangered Manglietia grandis Hu et Cheng[J]. Plant Physiology Communications,2009,45(11):1089-1092.
6. 刘延文,杨丽,焦冰清,等. 蓝靛果忍冬种子萌发特性研究[J]. 种子,2017,36(8):96-99.Liu Y W,Yang L,Jiao B Q,et al. Study on characteristics of seed germination of Lonicera caerulea L. [J]. Seed,2017,36(8):96-99.
7. Ahrendt L W A. Berberis and Mahonia:a taxonomic revision[J]. Botanical Journal of the Linnean Society,1961,57(369):1-410.
8. 杨柏珍. 小檗属(Berheris)植物的开发利用研究与保护[J]. 植物学报,1994,11(1):54-54.Yang B Z. Development and utilization research and protection of Berberis plants[J]. Journal of Botany,1994,11(1):54-54.
9. 李路扬,龙娓芳,万定荣,等. 5种小檗属药用植物总生物碱及小檗碱的含量测定[J]. 中国实验方剂学杂志,2014,20(17):91-94.Li L Y,Long W F,Wan D R,et al. Quantitative determination of total alkaloids and Berberine in five medicinal Berberis plants[J]. Chinese Journal of Experimental Traditional Medical Formulae,2014,20(17):91-94.
10. 阎国荣. 新疆天山野生果树研究及保护[J]. 中国野生植物资源,2001,20(4):13-14.Yan G R. Study on the wild fruit trees and its conservation of Tianshan mountain in Xinjiang[J]. Chinese Wild Plant Resources,2001,20(4):13-14.
11. 库尔班江,亥依如拉木,罗新泽. 伊犁河流域红果小檗资源分布与形态调查[J]. 中国野生植物资源,2007,26(5):26-30.Korbanjhon,Hayrullam,Luo X Z. Investigation on resource distribution and morphous condition of Berberis nummularia Bge. in Ili river's drainage basin[J]. Chinese Wild Plant Resources,2007,26(5):26-30.
12. 张婷婷,李莉,高宁,等. 新疆黑果小檗根部化学成分初步研究[J]. 时珍国医国药,2017,28(4):841-843.Zhang T T,Li L,Gao N,et al. Preliminary qualitative study of chemical constituents of Xinjiang Berberis heteropoda root[J]. Lishizhen Medicine and Materia Medica Research,2017,28(4):841-843.
13. 孙颖平,李莉. 新疆特色药材黑果小檗薄层鉴别方法研究[J]. 西北药学杂志,2013,28(3):231-234.Sun Y P,Li L. Study on thin-layer chromatography identification method for Xinjiang characteristic medicine, Berberis heteropoda Schrenk[J]. Northwest Pharmaceutical Journal,2013,28(3):231-234.
14. 冯云选. 种子贮藏加工[M]. 北京:化学工业出版社,2011.Feng Y X. Seed storage processing[M]. Beijing:Chemical Industry Press,2011.
15. 韩春艳,孙卫邦,董青松,等. 广州相思子和毛相思子种子活力TTC测定方法研究[J]. 种子,2011,30(7):13-16.Han C Y,Sun W B,Dong Q S,et al. Study on testing seed viability of Abrus cantoniensis and Abrus mollis with TTC method[J]. Seed,2011,30(7):13-16.
16. 卢明艳,杜研,毕晓颖,等. 5种野生无髯鸢尾种子休眠与萌发特性研究[J]. 西北植物学报,2017,37(9):1823-1830.Lu M Y,Du Y,Bi X Y,et al. Study on seed dormancy and germination characteristics with five species of wild Apogons Iris[J]. Acta Botanica Boreali-Occidentalia Sinica,2017,37(9):1823-1830.
17. 郎思睿,高逸超,赵航,等. 香叶树种子休眠与萌发特性的研究[J]. 北京林业大学学报,2011,33(6):124-129.Lang S R,Gao Y C,Zhao H,et al. Dormancy and germination characteristics of Lindera communis seed[J]. Journal of Beijing Forestry University,2011,33(6):124-129.
18. 司倩倩,臧德奎,刘丹,等. 玉铃花种子休眠原因及其内源激素含量变化[J]. 北方园艺,2017(6):91-95.Si Q Q,Zang D K,Liu D,et al. Causes of dormancy and change of endogenous hormone content in Styrax obassia seeds[J]. Northern Horticulture,2017(6):91-95.
19. 周芝琴,李廷山,胡小文. 莎草科4种植物种子休眠与萌发特性的研究[J]. 西北植物学报,2013,33(9):1885-1890.Zhou Z Q,Li T S,Hu X W. Seed dormancy and germination characteristics of four Cyperaceae species[J]. Acta Botanica Boreali-Occidentalia Sinica,2013,33(9):1885-1890.
20. 唐澄莹,张艳玲,汪云叶,等. 珍稀植物北碚榕的种子萌发及繁殖研究[J]. 西南大学学报:自然科学版,2017,39(2):34-39.Tang C Y,Zhang Y L,Wang Y Y,et al. Seed germination and propagation of the rare plant species Ficus beipeiensis[J]. Journal of Southwest University:Natural Science Edition,2017,39(2):34-39.
21. 翟书华,杨晓虹. 不同激素处理对海菜花种子萌发特性的影响[J]. 种子,2017,36(8):72-74.Zhai S H,Yang X H. Effects of different hormones treatments on seeds germination of Ottelia acuminata[J]. Seed,2017,36(8):72-74.

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不同天然居群小檗种子萌发障碍因子研究

Germination Obstacle Factors of Berberis Seeds of Different Natural Populations

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