干旱胁迫下拟南芥磷脂酶Dδ与一氧化氮在种子萌发中的信号关系

doi:10.7525/j.issn.1673-5102.2018.04.006

摘要/Abstract

摘要： 以拟南芥野生型（WT）、一氧化氮合酶（NOS）缺失型突变体（noa1）、硝酸还原酶（NR）缺失型突变体（nia1，nia2）及磷脂酶Dδ（PLDδ）缺失型突变体（pldδ）幼苗为材料，研究了0.3 mol·L^-1甘露醇模拟干旱胁迫响应过程中PLDδ和一氧化氮（NO）之间的信号转导关系。结果显示：干旱胁迫下NO含量，PLD和NR活性及基因相对表达量显著升高，pldδ和nia2较其他突变体对干旱胁迫更敏感；外源添加NO供体硝普钠（SNP）可以提高干旱胁迫下WT，nia2和pldδ的种子萌发，而外源添加磷脂酸（PA）可以促进WT和pldδ的种子萌发，但不能促进nia2的种子萌发；PA可以促进干旱胁迫下WT和pldδ的NO产生，但不能促进nia2中NO的产生。表明：干旱胁迫下PLDδ/PA位于NO信号的上游，且PLDδ/PA主要通过NR2途径产生的NO促进干旱胁迫下拟南芥的种子萌发。

关键词: 干旱胁迫, 磷脂酶Dδ, 一氧化氮, 硝酸还原酶

Abstract: With Arabidopsis wild-type(WT), nitric oxide synthase(NOS) deletion mutant(noa1), nitrate reductase(NR) deletion mutant(nia1 and nia2) and PLDδ deletion mutant(pldδ) of seedlings, we studied the relationship between Arabidopsis thaliana PLDδ and nitric oxide(NO) in response to the seed germination under drought stress. NO content, PLD and NR activity and gene relative expression were significantly increased under drought stress, while pldδ and nia2 are more sensitive than other mutants to drought stress. Sodium nitroprusside(SNP), as an exogenous NO donor, can improve the seed germination of WT, nia2 and pldδ. The exogenous phosphatidic acid(PA) can promote seed germination of WT and pldδ under drought stress except nia2. Besides, in compensation experiment, PA can promote the production of NO in WT and pldδ under drought stress, but not nia2 do. Therefore, it is speculated that PLDδ/PA located at the upstream of NO signal under drought stress, and the NO produced by NR2 is the main way for PLDδ/PA to promote Arabidopsis seed germination under drought stress.

Key words: drought stress, phospholipase Dδ, nitric oxide, nitrate reductase

中图分类号:

Q979.748.3

张旭强, 赵敏, 陈璐, 安炎黄, 杨鹏军, 王新霞, 杨宁. 干旱胁迫下拟南芥磷脂酶Dδ与一氧化氮在种子萌发中的信号关系[J]. 植物研究, 2018, 38(4): 526-534.

ZHANG Xu-Qiang, ZHAO Min, CHEN Lu, AN Yan-Huang, YANG Peng-Jun, Wang Xin-Xia, YANG Ning. Relationship Between Phospholipase Dδ and Nitric Oxide Responding to the Seed Germination in Arabidopsis thaliana under Drought Stress[J]. Bulletin of Botanical Research, 2018, 38(4): 526-534.

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