青藏高原特有种-藏扇穗茅叶绿体基因组测序及序列分析

doi:10.7525/j.issn.1673-5102.2018.04.005

植物研究 ›› 2018, Vol. 38 ›› Issue (4): 518-525.doi: 10.7525/j.issn.1673-5102.2018.04.005

青藏高原特有种-藏扇穗茅叶绿体基因组测序及序列分析

刘玉萍^1,2,3,4, 吕婷^1,2,3,4, 朱迪^1,2,3,4, 周勇辉^1,2,3,4, 刘涛^1,2,3,4, 苏旭^1,2,3,4

1. 青海师范大学生命科学学院, 西宁 810008;
2. 青海省青藏高原药用动植物资源重点实验室, 西宁 810008;
3. 青海省自然地理与环境过程重点实验室, 西宁 810008;
4. 青藏高原环境与资源教育部重点实验室, 西宁 810008

收稿日期:2018-01-23 出版日期:2018-07-15 发布日期:2018-07-21
通讯作者: 苏旭 E-mail:xusu8527972@126.com
作者简介:刘玉萍(1980-),女,博士,副教授,主要从事系统与进化植物学研究。
基金资助:
国家自然科学基金项目（41761009，31260052）；青海省自然科学基金项目（2017-ZJ-904）；青海省“高端创新人才千人计划”项目；青海省高等院校“135高层次人才培养工程”项目

Sequencing and Alignment Analysis of the Complete Chloroplast Genome of Littledalea tibetica,an Endemic Species from the Qinghai-Tibet Plateau

LIU Yu-Ping^1,2,3,4, LÜ Ting^1,2,3,4, ZHU Di^1,2,3,4, ZHOU Yong-Hui^1,2,3,4, LIU Tao^1,2,3,4, SU Xu^1,2,3,4

1. School of Life Science, Qinghai Normal University, Xining 810008;
2. Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province, Xining 810008;
3. Key Laboratory of Physical Geography and Environmental Process in Qinghai Province, Xining 810008;
4. Key Laboratory of Education Ministry on Environments and Resources in the Qinghai-Tibet Plateau, Xining 810008

Received:2018-01-23 Online:2018-07-15 Published:2018-07-21
Supported by:
Under the auspices of the National Natural Science Foundation of China(41761009,31260052);The National Natural Science Foundation of Qinghai Province(2017-ZJ-904);"High-end Innovative Talents Thousands of People Plan" in Qinghai Province;"135 High-level Personnel Training Project" in Qinghai Province

摘要/Abstract

摘要： 藏扇穗茅（Littledalea tibetica）是禾本科（Poaceae）雀麦族（Bromeae）中一个具有重要生态价值的多年生高山特有种，主要分布于青藏高原及其毗邻地区。本文采用基于第二代高通量测序平台的Illumina MiSeq技术，对青藏高原特有种—藏扇穗茅进行了叶绿体基因组测序，首次建立了雀麦族物种的标准测序流程；同时，以其近缘物种—黑麦草（Lolium perenne）的叶绿体基因组序列作为参考，组装获得它的叶绿体基因组序列。结果表明，藏扇穗茅叶绿体基因组序列全长136 852 bp，GC含量为38.5%，呈典型的四段式结构，其中大（LSC）、小（SSC）单拷贝区大小分别为80 970和12 876 bp，反向互补重复区（IR）大小为21 503 bp，共注释得到141个基因，包含95个蛋白编码基因、38个tRNA基因和8个rRNA基因，主要分布于大单拷贝区和小单拷贝区。同时，基于藏扇穗茅和其它30种禾本科植物叶绿体基因全序列构建的系统发育树显示，藏扇穗茅与早熟禾亚科中小麦族植物亲缘关系较近。

关键词: 禾本科, 扇穗茅属, 藏扇穗茅, 叶绿体基因组, Illumina测序, 系统分析, 青藏高原

Abstract: Littledalea tibetica is an alpine perennial species in the grass tribe Bromeae(Poaceae) that is endemic to the Qinghai-Tibet Plateau and adjacent regions. In the present study, we sequenced the complete chloroplast(cp) genome of L.racemosa on the Illumina MiSeq platform, which was obtained based on the reference cp genome of its related species, Lolium perenne. This study the first complete cp sequence for the Bromeae. The total genome size was 136 852 bp in length with 38.5% GC content. It contains a pair of inverted repeat regions(21 503 bp) separated by a large single copy region(80 970 bp) and a small single copy region(12 876 bp). Within the newly sequenced cp genome, we annotated a total of 141 genes, which included 95 protein-coding genes, 38 tRNAs genes, and 8 rRNAs genes. The most of genes occur within the single copy regions. We used the cp genome of L.tibetica and 30 other chloroplast genomes to perform a phylogenetic analysis, indicating that L.tibetica is closely related species in tribe Triticeae of subfamily Pooideae.

Key words: Poaceae, Littledalea, Littledalea tibetica, chloroplast genome, Illumina sequencing, phylogenetic analysis, Qinghai-Tibet Plateau

中图分类号:

Q949.71

刘玉萍, 吕婷, 朱迪, 周勇辉, 刘涛, 苏旭. 青藏高原特有种-藏扇穗茅叶绿体基因组测序及序列分析[J]. 植物研究, 2018, 38(4): 518-525.

LIU Yu-Ping, LÜ Ting, ZHU Di, ZHOU Yong-Hui, LIU Tao, SU Xu. Sequencing and Alignment Analysis of the Complete Chloroplast Genome of Littledalea tibetica,an Endemic Species from the Qinghai-Tibet Plateau[J]. Bulletin of Botanical Research, 2018, 38(4): 518-525.

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青藏高原特有种-藏扇穗茅叶绿体基因组测序及序列分析

Sequencing and Alignment Analysis of the Complete Chloroplast Genome of Littledalea tibetica,an Endemic Species from the Qinghai-Tibet Plateau

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