沙枣果总黄酮类化合物的富集研究

doi:10.7525/j.issn.1673-5102.2018.03.020

摘要/Abstract

摘要： 通过对9种型号大孔吸附树脂静态筛选，最终确定AB-8型大孔吸附树脂用于沙枣果总黄酮化合物的富集，并通过动态实验，确定沙枣果总黄酮最优富集条件为：样品浓度2.43 mg·mL^-1，上样速率2 BV·h^-1，乙醇浓度70%，洗脱速率2 BV·h^-1。在最优工艺条件下，沙枣果总黄酮浓度由9.73%增加到49.52%；其中金丝桃苷含量由0.53%增加到3.07%，提高5.79倍。本研究为沙枣果总黄酮的开发利用提供了理论依据。

关键词: 沙枣, 黄酮, 富集研究

Abstract: Based on the static screening experiments, AB-8 resin was determined to be the best resin for enriching flavonoid of Elaeagnus angustifolia from nine kinds of macro-porous resins. According to the dynamic experiment results, some enriching conditions were optimized, as follows:the sample concentration was 2.43 mg·mL^-1; the flow rate was 2 BV·h^-1; the ethanol concentration was 70%; the elution rate was 2 BV·h^-1. The purity of total flavonoids from E. angustifolia was increased by 5.09 times, from 9.73% to 49.52%. The purity of Hypericin was increased by 5.79 times, from 0.53% to 3.07%. The results of this research would provide the theoretical basis and data support for the development and utilization of total flavonoids in E. angustifolia.

Key words: Elaeagnus angustifolia, flavonoids, enrichment

中图分类号:

S143.8

张子东, 张圆圆, 孟永斌, 徐蕾, 贾楠, 项凤影, 路祺. 沙枣果总黄酮类化合物的富集研究[J]. 植物研究, 2018, 38(3): 467-474.

ZHANG Zi-Dong, ZHANG Yuan-Yuan, MENG Yong-Bin, XU Lei, JIA Nan, XIANG Feng-Ying, LU Qi. Flavonoids Enrichment from Elaeagnus angustifolia Fruit[J]. Bulletin of Botanical Research, 2018, 38(3): 467-474.

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