巴西橡胶树6个PP2C家族基因成员分子信息学与抗旱功能分析

doi:10.7525/j.issn.1673-5102.2017.05.013

植物研究 ›› 2017, Vol. 37 ›› Issue (5): 730-737.doi: 10.7525/j.issn.1673-5102.2017.05.013

巴西橡胶树6个PP2C家族基因成员分子信息学与抗旱功能分析

张冬¹, 李晓娜², 肖厚贞³, 陆燕茜¹, 张宇¹, 王萌¹

1. 海南省热带生物资源可持续利用重点实验室, 海南大学热带农林学院, 海口 570228;
2. 中国热带农业科学院科技信息研究所, 海口 570228;
3. 海南大学材料与化工学院, 海口 570228

收稿日期:2017-04-20 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 张宇,E-mail:yuzhang_rain@hainu.edu.cn E-mail:yuzhang_rain@hainu.edu.cn
作者简介:张冬(1992-),女,硕士研究生,主要从事分子植物学研究。
基金资助:
国家自然科学基金（31660187，31660204）；现代农业产业技术体系建设专项资金资助（CARS-34-BC1）；海南省研究生创新科研课题（Hys2016-25）

Bioinformatics and Drought Tolerance of PP2C Gene Family Members in Hevea brasiliensis Muell. Arg.

ZHANG Dong¹, LI Xiao-Na², XIAO Hou-Zhen³, LU Yan-Xi¹, ZHANG Yu¹, WANG Meng¹

1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228;
2. Institute of Scientific and Technical Information Catas, Haikou 570228;
3. College of Materials and Chemical Engineering, Hainan Univeersity, Haikou 570228

Received:2017-04-20 Online:2017-09-15 Published:2017-09-15
Supported by:
The National Natural Science Fund(31660187,31660204);The earmarker fund for China Agriculture Research System(CARS-34-BC1);Innovative research project of Hainan graduate students(Hys2016-25)

摘要/Abstract

摘要： PP2C是一类丝氨酸/苏氨酸残基蛋白磷酸酶，在高等植物ABA信号途径中起着重要的作用。为阐明巴西橡胶树中PP2C基因的结构与功能，本研究通过生物信息学方法，从橡胶树转录组数据库中鉴定并获得6个PP2C家族基因，均含有PP2CD、F1和F2亚族。通过qRT-PCR技术对6个PP2C家族基因进行了干旱处理下的差异表达分析，发现6个基因都不同程度上响应橡胶树干旱胁迫。本研究为探究PP2C基因在橡胶树抗干旱反应机制提供了理论依据。

关键词: 巴西橡胶树, PP2C基因, 生物信息学, 表达分析, 干旱

Abstract: PP2Cs are a group of monomeric serine/threonine protein phosphatases, which play an important role in ABA signaling pathway in higher plants. In order to elucidate the structure and function of PP2C gene in rubber tree,we used a bioinformatics approach to get six PP2C genes members from rubber tree transcription database, six HbPP2Cs all contain PP2C conserved domains.By sequence alignment and phylogenetic analysis,six HbPP2Cs were divided into four subfamilies:subfamily A, D, F1 and F2. Different expression analysis of six PP2C family genes under drought stress by qRT-PCR showed that they all response to drought stress in varying degrees.Our study provides a theoretical basis for exploring the mechanism of PP2C genes in drought resistance of rubber tree.

Key words: Hevea brasiliensis Muell.Arg., PP2C genes, bioinformatics, expression analysis, drought

中图分类号:

S794.1

张冬, 李晓娜, 肖厚贞, 陆燕茜, 张宇, 王萌. 巴西橡胶树6个PP2C家族基因成员分子信息学与抗旱功能分析[J]. 植物研究, 2017, 37(5): 730-737.

ZHANG Dong, LI Xiao-Na, XIAO Hou-Zhen, LU Yan-Xi, ZHANG Yu, WANG Meng. Bioinformatics and Drought Tolerance of PP2C Gene Family Members in Hevea brasiliensis Muell. Arg.[J]. Bulletin of Botanical Research, 2017, 37(5): 730-737.

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巴西橡胶树6个PP2C家族基因成员分子信息学与抗旱功能分析

Bioinformatics and Drought Tolerance of PP2C Gene Family Members in Hevea brasiliensis Muell. Arg.

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