超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化

doi:10.7525/j.issn.1673-5102.2017.03.020

植物研究 ›› 2017, Vol. 37 ›› Issue (3): 474-480.doi: 10.7525/j.issn.1673-5102.2017.03.020

• 研究简报 • 上一篇

超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化

倪玉娇¹, 赵春建¹, 李春英¹, 李国春², 王楷婷¹, 张玉坤¹

1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
2. 黑龙江省林业监测规划院, 哈尔滨 150040

收稿日期:2017-02-27 出版日期:2017-05-15 发布日期:2017-06-03
通讯作者: 李春英,E-mail:lcy@nefu.edu.cn E-mail:lcy@nefu.edu.cn
作者简介:倪玉娇(1992-),女,硕士研究生,主要从事植物资源开发与利用研究。
基金资助:
中央高校基本科研业务费专项资金项目（2572015EA04）

Process Optimization of Ultrasonic Assisted Deep Eutectic Solvents(DESs) Extraction of Polyphenols from Hippophae rhamnoides Seed Meal

NI Yu-Jiao¹, ZHAO Chun-Jian¹, LI Chun-Ying¹, LI Guo-Chun², WANG Kai-Ting¹, ZHANG Yu-Kun¹

1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
2. Heilongjiang Province Forestry Survey and Planning Institute, Harbin 150040

Received:2017-02-27 Online:2017-05-15 Published:2017-06-03
Supported by:
The Fundamental Research Funds for the Central Universities(2572015EA04)

摘要/Abstract

摘要： 以一系列低共熔溶剂为提取剂，采用超声波辅助法从沙棘籽粕中提取多酚。在单因素试验结果基础上，利用Box-Behnken实验设计，运用响应面分析法对影响沙棘籽粕多酚得率的主要因素（超声功率、超声时间、超声温度）进行优化。结果表明，沙棘籽粕多酚最佳提取工艺条件为：以含水量为30%的氯化胆碱—草酸低共熔溶剂为最佳提取剂，液料比为14∶1，超声功率420 W，超声时间56 min，超声温度44℃。在此条件下，多酚得率为3.31±0.008%。对比试验发现：氯化胆碱—草酸低共熔溶剂对沙棘籽粕多酚的得率明显优于传统溶剂；与热回流提取相比，超声提取法具有明显的优势。

关键词: 低共熔溶剂, 超声, 沙棘籽粕, 多酚

Abstract: A range of deep eutectic solvents(DESs) as the solvent were used for extraction polyphenols with ultrasonic-assisted extraction methods from sea buckthorn. According to the single-parameter study, experiment factors(ultrasonic power、ultrasonic time、ultrasonic temperature) remarkably affected the yield of polyphenols were selected and Box-Behnken experimental design was investigated to be optimized by response surface methodology. The results indicated that with 30% water in Choline chloride-Oxalic acid as the suitable solvent and liquid to material ratio of 14:1, the optimal conditions of ultrasonic extraction were as follows:ultrasonic power of 420 W, ultrasonic time of 56 min, and ultrasonic temperature of 44℃. Under these conditions, the yield of polyphenols was(3.31±0.08)%. Comparison experiment indicated that the extraction efficiency of polyphenols from sea buckthorn seed meal by DES was superior to the traditional solvent. Compared with the heat refluxing extraction method, DESs extraction method was a extraction technology with high efficiency.

Key words: deep eutectic solvents(DESs), ultrasonic-assisted extraction, sea buckthorn seed meal, polyphenol

中图分类号:

Q949.9

倪玉娇, 赵春建, 李春英, 李国春, 王楷婷, 张玉坤. 超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化[J]. 植物研究, 2017, 37(3): 474-480.

NI Yu-Jiao, ZHAO Chun-Jian, LI Chun-Ying, LI Guo-Chun, WANG Kai-Ting, ZHANG Yu-Kun. Process Optimization of Ultrasonic Assisted Deep Eutectic Solvents(DESs) Extraction of Polyphenols from Hippophae rhamnoides Seed Meal[J]. Bulletin of Botanical Research, 2017, 37(3): 474-480.

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超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化

Process Optimization of Ultrasonic Assisted Deep Eutectic Solvents(DESs) Extraction of Polyphenols from Hippophae rhamnoides Seed Meal

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