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植物研究 ›› 2017, Vol. 37 ›› Issue (3): 468-473.doi: 10.7525/j.issn.1673-5102.2017.03.019

• 研究报告 • 上一篇    下一篇

7个变异红叶杜仲叶片色素及活性成分分析

杜庆鑫1,2, 庆军1,2, 朱景乐1,2, 杜红岩1,2, 杜兰英1,2   

  1. 1. 中国林业科学研究院经济林研究开发中心, 郑州 450003;
    2. 国家林业局杜仲工程技术研究中心, 郑州 450003
  • 收稿日期:2017-01-06 出版日期:2017-05-15 发布日期:2017-06-03
  • 通讯作者: 杜兰英,E-mail:dly371@126.com E-mail:dly371@126.com
  • 作者简介:杜庆鑫(1991-),男,硕士研究生,主要从事杜仲育种与栽培。
  • 基金资助:
    国家“十二五”科技支撑计划(2012BAD21B0502)

Pigments and Active Ingredients in Leaves of Seven Eucommia ulmoides Variation-types

DU Qing-Xin1,2, QING Jun1,2, ZHU Jing-Le1,2, DU Hong-Yan1,2, DU Lan-Ying1,2   

  1. 1. Non-timber Forest Research and Development Center of Chinese Academy of Forestry, Zhengzhou 450003;
    2. The Eucommia Engineering Research Center of National Forestry Administration, Zhengzhou 450003
  • Received:2017-01-06 Online:2017-05-15 Published:2017-06-03
  • Supported by:
    National "twelve five" scienticific and technological project(2012BAD21B0502)

摘要: 为探讨不同变异类型红叶杜仲叶片呈色机理及活性成分含量,以7个不同变异红叶杜仲和绿叶杜仲为材料,对叶片颜色、色素分布、含量及活性成分含量进行测定分析。结果表明:7个变异红叶杜仲叶片正面呈暗红或紫红色,栅栏组织呈红色或暗红色;背面颜色3、5和6号呈红色,下表皮也呈红色,1、2、4和7号为绿色,下表皮透明;7个变异红叶杜仲叶片CaCbCT含量均低于对照杜仲叶片,而CCarCF含量以及CCar/CTCF/CT值均显著高于对照叶片,叶片色素含量在7个红叶杜仲间也存在一定差异;7个变异红叶杜仲叶片5种活性成分含量均较高,且绿原酸、京尼平苷和总黄酮含量明显高于对照杜仲叶片。叶片中CF分布、含量及CF/CT值是红叶杜仲呈色差异的主要原因,高活性成分含量使其在药用方面有很大的开发利用潜力。本研究可为观赏兼药用杜仲资源选育提供材料。

关键词: 红叶杜仲, 叶片, 色素, 活性成分

Abstract: The experiment was conducted to investigate the formation reasons for different colors and the active ingredients content of red leaf Eucommia ulmoides variation-types. With seven red leaf E.ulmoides variation-types and one green leaf E.ulmoides, the color, pigments distribution and content, and active ingredients content in leaf were determined. The front of blade was dark red or purple red of seven red leaf E.ulmoides variation-types, and the palisade tissue was red or dark red. The backs of No.3, 5 and 6 were red with red lower epidermis, and those of No.1, 2, 4 and 7 were green with transparent lower epidermis. The contents of Ca, Cb and CT in seven red leaf were lower than those of in control check leaf, while CCar, CF content and the value of CCar/CT and CF/CT were significantly higher than those of in control check leaf, and there were the differences of pigment content between seven red leaf. Five kinds of active ingredients content were high in seven red lea, and the contents of chlorogenic acid, geniposide, and total flavonoids were higher than those of in control check. The distribution and content of CF and the value of CF/CT were the main reasons for different colors of seven red leaf, and the high content of active ingredients made it of great potential in the utilization of medicine. This study would provide materials for ornamental and medicine E.ulmoides resources.

Key words: red leaf Eucommia ulmoides variation-types, leaf, pigments, active ingredients

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