7个变异红叶杜仲叶片色素及活性成分分析

doi:10.7525/j.issn.1673-5102.2017.03.019

植物研究 ›› 2017, Vol. 37 ›› Issue (3): 468-473.doi: 10.7525/j.issn.1673-5102.2017.03.019

7个变异红叶杜仲叶片色素及活性成分分析

杜庆鑫^1,2, 庆军^1,2, 朱景乐^1,2, 杜红岩^1,2, 杜兰英^1,2

1. 中国林业科学研究院经济林研究开发中心, 郑州 450003;
2. 国家林业局杜仲工程技术研究中心, 郑州 450003

收稿日期:2017-01-06 出版日期:2017-05-15 发布日期:2017-06-03
通讯作者: 杜兰英,E-mail:dly371@126.com E-mail:dly371@126.com
作者简介:杜庆鑫(1991-),男,硕士研究生,主要从事杜仲育种与栽培。
基金资助:
国家“十二五”科技支撑计划（2012BAD21B0502）

Pigments and Active Ingredients in Leaves of Seven Eucommia ulmoides Variation-types

DU Qing-Xin^1,2, QING Jun^1,2, ZHU Jing-Le^1,2, DU Hong-Yan^1,2, DU Lan-Ying^1,2

1. Non-timber Forest Research and Development Center of Chinese Academy of Forestry, Zhengzhou 450003;
2. The Eucommia Engineering Research Center of National Forestry Administration, Zhengzhou 450003

Received:2017-01-06 Online:2017-05-15 Published:2017-06-03
Supported by:
National "twelve five" scienticific and technological project(2012BAD21B0502)

摘要/Abstract

摘要： 为探讨不同变异类型红叶杜仲叶片呈色机理及活性成分含量，以7个不同变异红叶杜仲和绿叶杜仲为材料，对叶片颜色、色素分布、含量及活性成分含量进行测定分析。结果表明：7个变异红叶杜仲叶片正面呈暗红或紫红色，栅栏组织呈红色或暗红色；背面颜色3、5和6号呈红色，下表皮也呈红色，1、2、4和7号为绿色，下表皮透明；7个变异红叶杜仲叶片C_a、C_b和C_T含量均低于对照杜仲叶片，而C_Car、C_F含量以及C_Car/C_T、C_F/C_T值均显著高于对照叶片，叶片色素含量在7个红叶杜仲间也存在一定差异；7个变异红叶杜仲叶片5种活性成分含量均较高，且绿原酸、京尼平苷和总黄酮含量明显高于对照杜仲叶片。叶片中C_F分布、含量及C_F/C_T值是红叶杜仲呈色差异的主要原因，高活性成分含量使其在药用方面有很大的开发利用潜力。本研究可为观赏兼药用杜仲资源选育提供材料。

关键词: 红叶杜仲, 叶片, 色素, 活性成分

Abstract: The experiment was conducted to investigate the formation reasons for different colors and the active ingredients content of red leaf Eucommia ulmoides variation-types. With seven red leaf E.ulmoides variation-types and one green leaf E.ulmoides, the color, pigments distribution and content, and active ingredients content in leaf were determined. The front of blade was dark red or purple red of seven red leaf E.ulmoides variation-types, and the palisade tissue was red or dark red. The backs of No.3, 5 and 6 were red with red lower epidermis, and those of No.1, 2, 4 and 7 were green with transparent lower epidermis. The contents of C_a, C_b and C_T in seven red leaf were lower than those of in control check leaf, while C_Car, C_F content and the value of C_Car/C_T and C_F/C_T were significantly higher than those of in control check leaf, and there were the differences of pigment content between seven red leaf. Five kinds of active ingredients content were high in seven red lea, and the contents of chlorogenic acid, geniposide, and total flavonoids were higher than those of in control check. The distribution and content of C_F and the value of C_F/C_T were the main reasons for different colors of seven red leaf, and the high content of active ingredients made it of great potential in the utilization of medicine. This study would provide materials for ornamental and medicine E.ulmoides resources.

Key words: red leaf Eucommia ulmoides variation-types, leaf, pigments, active ingredients

中图分类号:

Q949.751.5

杜庆鑫, 庆军, 朱景乐, 杜红岩, 杜兰英. 7个变异红叶杜仲叶片色素及活性成分分析[J]. 植物研究, 2017, 37(3): 468-473.

DU Qing-Xin, QING Jun, ZHU Jing-Le, DU Hong-Yan, DU Lan-Ying. Pigments and Active Ingredients in Leaves of Seven Eucommia ulmoides Variation-types[J]. Bulletin of Botanical Research, 2017, 37(3): 468-473.

参考文献

1. 杜红岩,胡文臻,俞锐. 杜仲产业绿皮书:中国杜仲橡胶资源与产业发展报告[M]. 北京:社会科学文献出版社,2013:1-3.Du H Y,Hu W Z,YU R. Green book of Eucommia industry:The report on development of China's Eucommia rubber resources and industry[M]. Beijing:Social Sciences Academic Press,2013,1-3.
2. 吴敏,赵阳,马志刚,等. 果园化栽培模式杜仲雄花、果实和叶片产量的调查分析[J]. 林业科学研究,2014,27(2):270-276.Wu M,Zhao Y,Ma Z G,et al. Investigation of male flower,fruit and leaf yield from an Eucommia ulmoides orchard system[J]. Forest Research,2014,27(2):270-276.
3. 王璐,杜兰英,杜红岩. 杜仲饲料添加剂的研究进展[J]. 饲料研究,2014(19):29-31.Wang L,Du L Y,Du H Y. Research progress on feed additives of Eucommia ulmoides[J]. Feed Research,2014(19):29-31.
4. 魏艳秀,刘攀峰,杜庆鑫,等. 不同种质杜仲叶中多酚和黄酮含量差异性分析[J]. 林业科学研究,2016,29(4):529-535.Wei Y X,Liu P F,Du Q X,et al. Comparison in contents of polyphenol and flavonoid in leaves of Eucommia ulmoides germplasm[J]. Forest Research,2016,29(4):529-535.
5. 杜红岩. 中国杜仲图志[M]. 北京:中国林业出版社,2014:196-206.Du H Y. China Eucommia pictorial[M]. Beijing:China Forestry Publishing House,2014:196-206.
6. 张博勇,张康健,张檀,等. 秦仲1-4号优良品种选育研究[J]. 西北林学院学报,2004,19(3):18-20.Zhang B Y,Zhang K J,Zhang T,et al. Researches on the selection and breeding of superior species of Qizhong 1-4[J]. Journal of Northwest Forestry University,2004,19(3):18-20.
7. 张书敏,刘红云,程金金,等. 快速徒手切片法观察谷子和水稻叶片显微结构[J]. 基因组学与应用生物学,2015,34(7):1527-1530.Zhang S M,Liu H Y,Cheng J J,et al. Free-hand section techniques for clear observation of cell structures of millet(Setaria italica) and rice(Oyrza sativa) leaves[J]. Genomics and Applied Biology,2015,34(7):1527-1530.
8. 黄帆,郭正元,徐珍. 测定浮萍叶绿素含量的方法研究[J]. 实验技术与管理,2007,24(5):29-31.Huang F,Guo Z Y,Xu Z. Determined methods of chlorophyll from Lemma paucicostata[J]. Experimental Technology and Management,2007,24(5):29-31.
9. 朱景乐,李芳东,杜红岩,等. 3个观赏型杜仲无性系叶片色素含量比较[J]. 林业科学研究,2014,27(4):562-564.Zhu J L,Li F D,Du H Y,et al. Comparison of leaf pigment content among three ornamental Eucommia ulmoides varieties[J]. Forest Research,2014,27(4):562-564.
10. 范杰平. 柿叶中有效成分的提取与分离研究[D]. 杭州:浙江大学,2006:83-86.Fan J P. Study on the extraction and separation of the active components in the leaves of Diospyros kaki[D]. Hangzhou:Zhejiang University,2006:83-86.
11. 郭洋静,丁艳霞,许兰波,等. HPLC法同时测定杜仲雄花中5种活性成分[J]. 中成药,2014,36(10):2131-2134.Guo Y J,Ding Y X,Xu L B,et al. Simultaneous determination of five compounds in male flowers of Eucommia ulmoids Oliv by HPLC[J]. Chinese Traditional Patent Medicine,2014,36(10):2131-2134.
12. 朱书香,杨建民,王中华,等. 4种李属彩叶植物色素含量与叶色参数的关系[J]. 西北植物学报,2009,29(8):1663-1669.Zhu S X,Yang J M,Wang Z H,et al. Relationship between pigment contents and leafcolor parameters of four leaf-colored species of Prunus[J]. Acta Botanica Boreali-Occidentalia Sinica,2009,29(8):1663-1669.
13. 李亚蒙,王庆菊,沈向. 4种李属彩叶树种枝叶花色苷分布的解剖研究[J]. 山东农业大学学报:自然科学版,2006,37(4):489-494.Li Y M,Wang Q J,Shen X. Pigment distribution in fresh shoot tissues of four colored-leaf Prunus taxa[J]. Journal of Shandong Agricultural University:Natural Science Edition,2006,37(4):489-494.
14. 张敏,黄利斌,周鹏,等. 榉树秋季转色期叶色变化的生理生化[J]. 林业科学,2015,51(8):44-51.Zhang M,Huang L B,Zhou P,et al. Physiological and biochemical changes in Zelkova serrata leaves during leaf color transformation in autumn[J]. Scientia Silvae Sinicae,2015,51(8):44-51.
15. 胡敬志,田旗,鲁心安. 枫香叶片色素含量变化及其与叶色变化的关系[J]. 西北农林科技大学学报:自然科学版,2007,35(10):219-223.Hu J Z,Tian Q,Lu X A. Relationship between changes of the pigments content and leaf color changing in Liquidambar formosana[J]. Journal of Northwest A&F University:Natural Science Edition,2007,35(10):219-223.

[1]	隋德宗, 王保松. 盐胁迫下乌桕无性系叶片的比较蛋白组学研究[J]. 植物研究, 2023, 43(5): 679-689.
[2]	庄舒尧, 许恒博, 胡骁彧, 戴上, 张彦妮. 盐碱胁迫对彩叶矾根‘银扇’幼苗生长和生理特性的影响[J]. 植物研究, 2023, 43(4): 520-530.
[3]	李浙浙, 张一丹, 王波, 王郑昊, 王璐, 程蓓蓓. 木槿开花过程中花色素的变化规律及其影响因素[J]. 植物研究, 2023, 43(4): 550-561.
[4]	康莹, 潘晶晶, 陈建华, 刘权钢, 董胜君. 金叶复叶槭叶片色素与解剖结构[J]. 植物研究, 2023, 43(4): 591-600.
[5]	李婷婷, 杨柳, 李晓霞, 王奕淞, 王秀伟. 水曲柳和蒙古栎光合特性和生长对不同氮素形态的响应[J]. 植物研究, 2023, 43(2): 207-217.
[6]	刘阳, 许丽颖, 魏统超, 申澜懿, 刘斗南, 刘月. 4种槭树叶功能性状及其关系对季节变化的响应[J]. 植物研究, 2023, 43(2): 242-250.
[7]	周兵, 闫小红, 雷艺涵, 彭超, 陈丽花. 紫茉莉开花过程中花色和花色素的变化规律[J]. 植物研究, 2022, 42(3): 475-482.
[8]	盖庆岩, 王紫莹, 付玉杰, 焦骄, 王慧梅, 鹿瑶, 刘婧, 富锦先, 徐晓杰, 姚兰. UV-B辐射对84K杨酚酸类化合物积累及其生理生化的影响[J]. 植物研究, 2021, 41(6): 878-887.
[9]	胡文海, 肖宜安, 闫小红, 叶子飘, 曾建军, 李晓红. 越冬期红叶石楠和桂花防御低温强光伤害的光保护机制[J]. 植物研究, 2021, 41(6): 938-946.
[10]	何凤, 杜红岩, 刘攀峰, 王璐, 庆军, 杜兰英. 干旱胁迫对杜仲叶片结构特征的影响[J]. 植物研究, 2021, 41(6): 947-956.
[11]	袁秀云, 许申平, 周一冉, 王喜蒙, 崔波. 遮荫对白及形态及叶片结构的影响[J]. 植物研究, 2021, 41(6): 974-981.
[12]	李钰, 杨晓, 叶霄, 邓洁琼, 黄位年, 代沙. 超声辅助提取红花中主要活性成分的工艺优化[J]. 植物研究, 2021, 41(5): 824-835.
[13]	张书齐, 许全, 姚海荣, 杨秋, 刘文杰, 王萌. 海南岛海岸带木麻黄和厚藤叶片碳、氮、磷含量及其化学计量特征[J]. 植物研究, 2020, 40(2): 224-232.
[14]	刘旻霞, 李俐蓉, 车应弟, 焦骄. 高寒草甸不同演替阶段植物叶片功能性状研究[J]. 植物研究, 2019, 39(5): 760-769.
[15]	胡举伟, 代欣, 宋涛, 孙广玉. 不同光质对桑树幼苗生长和光合特性的影响[J]. 植物研究, 2019, 39(4): 481-489.

7个变异红叶杜仲叶片色素及活性成分分析

Pigments and Active Ingredients in Leaves of Seven Eucommia ulmoides Variation-types

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价