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植物研究 ›› 2000, Vol. 20 ›› Issue (2): 201-206.

• 论文 • 上一篇    下一篇

凉水国家自然保护区天然红松林遗传变异的RAPD分析

张恒庆1, 安利佳3, 祖元刚2   

  1. 1. 辽宁师范大学生物系, 大连 116029;
    2. 东北林业大学森林植物生态学开放研究实验室, 哈尔滨 150040;
    3. 大连理工大学生物工程系, 大连 116012
  • 收稿日期:1999-07-29 出版日期:2000-06-15 发布日期:2016-06-14

RAPD ANALYSIS ON GENETIC VARIATION OF PINUS KORAIENSIS IN LIANG SHUI NATIONAL NATURAL RESERVE

ZHANG Heng-qing1, AN Li-jia3, ZU Yuan-gang2   

  1. 1. Department of Biology LiaoNing Normal University Dalian 1 16029;
    2. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040;
    3. Department of Bioengineering, Dalian 116012
  • Received:1999-07-29 Online:2000-06-15 Published:2016-06-14

摘要: 采用随机扩增多态DNA(random amplified polymorphic DNA RAPD)技术,研究了凉水国家自然保护区天然红松林的遗传变异水平及其分布规律。16个10bp长度的随机引物在8个实验样地内72个个体中共检测了96个位点,其中55个是多态位点。在种水平上,红松的多态位点比率为P=58.51%;Nei's遗传变异指数为H=0.2868;Shannon's指数为I=0.3654,所有的变异中,有92.47%的变异存在于样地内, 7.53%的变异存在于样地间。根据各遗传多样性在不同样地的分布,初步探讨了红松遗传变异水平与其种群发生及生境的相互关系。

关键词: 红松, RAPD, 遗传变异, 遗传多样性

Abstract: The genetic variation and the regulation of genetic diversity distribution of Pinus koraiensis were studied by random amplified polymorphic DNA (RAPD) analysis. 72 individuals come from 8 plots in Liang Shui natural reserve were amplified by PCR reaction. A total of 96 loci were obtained from 1 6 random primers, among which 55 loci were polymorphic. In species level, the percentage of polymorphic loci is P=58.51%, Nei's gene diversity is H=0.2868 and Shannon's information index is I=0.3654. The degree of genetic differentiation among 8 plots Gst=0.0753. According to the characterized of every plot, relation ship between the level of genetic variation and character of population habitat were discussed.

Key words: Pinus koraiensis, RAPD, genetic variation, genetic diversity