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植物研究 ›› 2002, Vol. 22 ›› Issue (3): 322-327.

• 论文 • 上一篇    下一篇

菜豆种质资源RAPD多样性的研究Ⅱ

栾非时1, 祖元刚2   

  1. 1. 东北农业大学, 哈尔滨 150030;
    2. 东北林业大学, 哈尔滨 150040
  • 收稿日期:2001-02-16 出版日期:2002-09-15 发布日期:2016-06-14
  • 作者简介:栾非时(1964-),女,副教授,博士,主要从事蔬菜分子生物学、蔬菜专家系统、设施园艺方向的研究。本文为作者在博士后工作站期间研究的内容。

RAPD DIVERSITY OF GERMPLASM RESOURCES ON PHASEOLUS VULGARIS

LUAN Fei-shi1, ZU Yuan-gang2   

  1. 1. Northeast Agricultural University, Harbin 150030;
    2. Northeast Forestry University, Harbin 150040
  • Received:2001-02-16 Online:2002-09-15 Published:2016-06-14

摘要: 本研究收集了我国43个栽培品种,国际热带农业中心13个半野生品种,波兰4个矮生品种共60个菜豆品种资源,将其分成三大类型,即蔓生种35个,矮生种12个,半野生种13个,从RAPD标记上进行了研究,探明种内及各种群间的遗传相似度、遗传距离,绘制聚类分析图。综合RAPD标记聚类图,表明:蔓生种群分为五大类;矮生种群分为二大类;半野生种群分为二大类。我国是菜豆的主要育种、栽培地区,收集国内外菜豆种质资源,开展DNA水平的分子标记将有助于了解菜豆种内、各种群间的遗传基础,确定各资源材料间的亲缘关系,为我国菜豆种质资源的保存及培育优良品种工作提供一定的理论依据。

关键词: 菜豆, RAPD, 遗传多样性

Abstract: Sixty varieties of Phaseolus vulgaris were collected in this study, including 43 from Heilongjiang Province, 13 from The Center of International Agriculture Tropic and 4 from Poland, which were classified as 35 for vine, 12 for dwarf and 13 for semi-wild variety. This study was to analyze the genetic similarity and genetic distance in population and among populations of the 60 varieties and to draw the dendrogram of cluster analysis by using the markers of RAPD. Total 60 varieties in this study could be classified into 5 groups for vine population, 2 for dwarf population and 2 for semi-wild population based on the analysis of the dendrogram of cluster analysis for the markers of RAPD. It is of importance to collect Phaseolus vulgaris resources from home and abroad since Heilongjiang Province is the main area for breeding and cultivating Phaseolus vulgaris in China. The analysis for the markers of RAPD would be beneficial for investigating the genetic background of Phaseolus vulgaris in population and among populations and for identifying the inter-relationship in population. The present study would be valuable for preserving the germplasm and breeding good strains of Phaseolus vulgaris in Heilongjiang Province.

Key words: Phaseolus vulgaris, RAPD, genetic diversity