欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2005, Vol. 25 ›› Issue (2): 192-196.doi: 10.7525/j.issn.1673-5102.2005.02.018

• 论文 • 上一篇    下一篇

凉水、丰林国家自然保护区红松居群遗传多样性的RAPD分析

吕建洲, 吴隆坤, 刘德利, 李岩, 裴赢, 张恒庆   

  1. 辽宁师范大学生命科学学院, 大连 116029
  • 收稿日期:2004-08-07 出版日期:2005-06-15 发布日期:2016-06-14
  • 通讯作者: 张恒庆 E-mail:hengqingzhang@163.com
  • 作者简介:吕建洲(1957-),男,副教授,主要从事植物保护工作研究工作。
  • 基金资助:
    国家自然科学基金(30170176)资助项目

Comparison of genetic diversity of Pinus koraiensis in two national nature reserves by RAPD marker

LÜ Jian-Zhou, WU Long-Kun, LIU De-Li, LI Yan, PEI Ying, ZHANG Heng-Qing   

  1. College of Life Science, Liaoning Normal University, Dalian 116029
  • Received:2004-08-07 Online:2005-06-15 Published:2016-06-14

摘要: 采用了RAPD分子标记技术分析了凉水和丰林国家自然保护区红松(Pinus koraiensis)居群的遗传多样性。10个随机引物检测到61个可重复的位点,多态位点比率为0.6935。Nei指数统计结果表明,红松的遗传多样性凉水居群(0.2634)大于丰林居群(0.2607)。对比分析了这两个天然红松居群的遗传多样性变化,红松种内的遗传变异主要存在于居群内,居群间存在一定的遗传分化。

关键词: 红松, 遗传多样性, RAPD

Abstract: With RAPD method, the paper studied genetic diversity and differentiation of two natural populations of Pinus koraiensis in Liangshui National Nature Reserve and Fenglin National Nature Reserve. Through the amplification of RAPD with 10 random primers, 61 repeatable loci were detected, in the amplification and percentage of polymophic loci was 0.693 5%. Nei's index showed the levels of genetic diversity of Liangshui population(0.263 4) was higher than Fenglin'(0.260 7); genetic variation existed mainly within populations, there were little genetic differentiation between populations.

Key words: Pinus koraiensis, genetic diversity, RAPD