黄芩ICS基因部分编码序列的克隆及其对水杨酸含量影响的研究

doi:10.7525/j.issn.1673-5102.2016.03.016

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 421-426.doi: 10.7525/j.issn.1673-5102.2016.03.016

黄芩ICS基因部分编码序列的克隆及其对水杨酸含量影响的研究

苏虎^1,2, 朱友林¹, 李蓉², 吴超², 张芸²

1. 南昌大学生命科学学院, 南昌 330031;
2. 江西科技师范大学生命科学学院, 南昌 330013

收稿日期:2015-10-23 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 朱友林,E-mail:ylzhu1999@aliyun.com E-mail:ylzhu1999@aliyun.com
作者简介:苏虎(1978-),男,博士研究生,主要从事植物次生代谢研究。
基金资助:
国家自然科学基金项目(81102803);江西省研究生创新基金项目(YC2014-B013)

Cloning the Partial Coding Sequence of ICS Gene and the Influence of ICS Gene on the SA Content in Scutellaria baicalensis Georgi

SU Hu^1,2, ZHU You-Lin¹, LI Rong², WU Chao², ZHANG Yun²

1. Nanchang University, Nanchang 330031;
2. Jiangxi Science and Technology Normal University, Nanchang 330031

Received:2015-10-23 Online:2016-05-15 Published:2016-05-20

摘要/Abstract

摘要： 以黄芩为实验材料,利用PCR克隆了其异分支酸合成酶(Isochorismate synthase,ICS)基因的部分编码序列,利用该序列构建了病毒诱导的基因沉默(Virus induced gene silence,VIGS)载体pTRV2-VIGSics,对黄芩ICS基因进行了沉默;测定了沉默后ICS基因的表达水平及ICS酶活性,研究了ICS基因沉默对黄芩水杨酸(Salicylic acid,SA)含量的影响,以及干旱胁迫对沉默材料ICS基因表达水平、酶活性和SA含量的影响。结果表明VIGS沉默处理使ICS表达水平显著降低,为对照组的25%,ICS酶活性未检测到,黄芩SA含量为对照组的44.4%;干旱胁迫下ICS表达水平和酶活性有所恢复,分别为对照组的87%和4.95%,黄芩SA含量没有恢复,而是降低至了对照组的13.2%。

关键词: 黄芩, 异分支酸合成酶, 水杨酸, 病毒诱导的基因沉默

Abstract: We cloned partial coding sequences of isochorismate synthase(ICS) gene in Scutellaria baicalensis Georgi, and constructed the virus induced gene silence(VIGS) vector, pTRV2-VIGSics. ICS gene was silenced by VIGS technique. We analyzed the ICS activity, expression level, and salicylic acid content of the silenced seedlings. ICS silence resulted in the drastically reduction of the expression level, enzyme activity and salicylic acid content in S.baicalensis Georgi. The enzyme activity was not detected, the expression level was reduced to 25%, and salicylic acid content was reduced to 44.4%. While, drought stress caused the expression level and enzyme activity recovered to 87% and 4.95%, respectively. Instead of recovery, the salicylic acid content was further reduced to 13.2% of the wild type level under drought stress.

Key words: Scutellaria baicalensis Georgi, isochorismate synthase, salicylic acid, virus induced gene silence

中图分类号:

Q946.82+8.3

苏虎, 朱友林, 李蓉, 吴超, 张芸. 黄芩ICS基因部分编码序列的克隆及其对水杨酸含量影响的研究[J]. 植物研究, 2016, 36(3): 421-426.

SU Hu, ZHU You-Lin, LI Rong, WU Chao, ZHANG Yun. Cloning the Partial Coding Sequence of ICS Gene and the Influence of ICS Gene on the SA Content in Scutellaria baicalensis Georgi[J]. Bulletin of Botanical Research, 2016, 36(3): 421-426.

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黄芩ICS基因部分编码序列的克隆及其对水杨酸含量影响的研究

Cloning the Partial Coding Sequence of ICS Gene and the Influence of ICS Gene on the SA Content in Scutellaria baicalensis Georgi

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