干旱胁迫条件下转LbDREB基因大青杨瞬时基因表达及生长、生理指标变异分析

doi:10.7525/j.issn.1673-5102.2016.03.014

摘要/Abstract

摘要： 利用转基因技术培育杨树抗逆品种是林木分子育种的重要手段之一。本研究以3个转LbDREB基因株系大青杨及野生型WT为材料,利用PEG6000模拟干旱胁迫处理,调查胁迫条件下不同株系生长、瞬时LbDREB基因表达量和生化指标的变化。结果表明:随着PEG胁迫时间的增加,转基因大青杨较WT的生物量向根部分配更多;LbDREB基因表达量与SOD,POD变化趋势相近,呈现先上升后下降再上升的趋势;转基因株系较WT保护酶活性更强,脯氨酸含量积累增多,MDA积累降低,WT相对电导率比各转基因株系高;结果表明各转LbDREB基因株系的抗旱能力均优于WT,且LbDREB基因上调表达可能是调控植物抗旱能力的重要因素。转基因株系Dr2在干旱胁迫下各抗旱指标均表现优秀,可以初步筛选为大青杨抗旱优良无性系。

关键词: 大青杨, LbDREB基因, 旱胁迫, 瞬时表达

Abstract: We used transgenic technology to cultivate stress-resistant varieties of Populus ussuriensis Kom, which was one of the most significant means of molecular breeding.With three expressed LbDREB lines and one wild type(WT), under the condition of PEG6000, which simulated the treatment of the drought stress, we investigated the growth of different lines, and the relative expression levels of LbDREB and biochemical indicators.With the increase of PEG stress time, the root phytomass of expressed LbDREB line was more than wild type.Meanwhile, the variational trends of SOD and POD were similar to the LbDREB expression level, which presented the upward trend, the downward trend and eventually beingrising.Additionally, transgenic lines contained stronger protective enzymes activity and proline content than wild type.However, the MDA accumulation and relative conductivity of transgenic lines were less than wild type.The drought tolerance ability of transgenic lines was superior to wild type, furthermore, the up-regulated expression of LbDREB probably played an important role in drought resistant in plant.The transgenic line named Dr2 was perfect in the different index of drought resistant, which could be preliminary screened as the excellent drought resistant clones of P.ussuriensis Kom.

Key words: Populus ussuriensis Kom., LbDREB gene, drought stress, transient expression

中图分类号:

S792.113

李墨野, 王娜, 周宇, 普潇莹, 冯万举, 李开隆. 干旱胁迫条件下转LbDREB基因大青杨瞬时基因表达及生长、生理指标变异分析[J]. 植物研究, 2016, 36(3): 409-415.

LI Mo-Ye, WANG Na, ZHOU Yu, PU Xiao-Ying, FENG Wan-Ju, LI Kai-Long. Variation Analysis of Transient Gene Expression and Growth, Physiological Indicators under Drought Stress in Expressed LbDREB Populus ussuriensis Kom.[J]. Bulletin of Botanical Research, 2016, 36(3): 409-415.

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