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植物研究 ›› 2006, Vol. 26 ›› Issue (4): 430-434.doi: 10.7525/j.issn.1673-5102.2006.04.022

• 论文 • 上一篇    下一篇

离体条件下几种不同的因子对芦笋诱导生根的研究

吴翠蓉;刘丽娜;于爱萍;赵小梅;周根余*   

  1. (上海师范大学生命与环境科学学院生物系遗传学实验室,上海 200234)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-07-20 发布日期:2006-07-20
  • 通讯作者: 周根余
  • 基金资助:
     

Different factors on formation of rooting in Asparagua officinalis in vitro

WU Cui-Rong;LIU Li-Na;YU Ai-Ping;ZHAO Xiao-Mei;ZHOU Gen-Yu*   

  1. (College of Life and Environment Science, Shanghai Teachers University, Shanghai 200234)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: ZHOU Gen-Yu
  • Supported by:
     

摘要: 以芦笋的茎尖为外植体,分别从糖、无机盐、激素等方面研究了在离体条件下影响芦笋生根的因素。结果分析表明:1/2 MS、20 g·L-1的糖有利于生根;10 g·L-1糖、1/4 MS培养基上预处理12 d后,生根率可达到100%,根的质量明显优于生长在含有激素培养基上的根;激素预处理并不能提高生根率。因此,低盐及低糖预处理是提高芦笋生根率的一种比较好的方法。

关键词: 芦笋, 糖, 无机盐, 生根

Abstract: Using Asparagua officinalis stem shot as explant, the paper discussed the factors that affected the rooting in vitro. The concentration of sucrose, abiosalt and plant growth regulator were studied. The result indicated that 1/2MS and 20g·L-1 sucrose promoted rooting;Rooting rate arrived at 100% when it was pretreated in the twelfth with 1/4MS and 20 g·L-1 sucrose. Its quality of root excelled the root in the medium with hormone;pretreatment of hormone couldn’t enhance rooting rate.Therefore,low salt or low sucrose pretreatment was a kind of good way for advancing rate of rooting in Asparagua officinalis.

Key words: Asparagua officinalis, sucrose, abiosalt, rooting

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