胡麻资源萌发期耐盐综合性评价

doi:10.7525/j.issn.1673-5102.2019.06.019

植物研究 ›› 2019, Vol. 39 ›› Issue (6): 955-963.doi: 10.7525/j.issn.1673-5102.2019.06.019

胡麻资源萌发期耐盐综合性评价

赵玮¹, 齐燕妮¹, 张建平¹, 赵利¹, 王利民¹, 谢亚萍¹, 王斌¹, 张艳萍²

1. 甘肃省农业科学院作物研究所, 兰州 730070;
2. 甘肃省农业科学院生物技术研究所, 兰州 730070

收稿日期:2019-05-31 出版日期:2019-11-05 发布日期:2019-11-16
通讯作者: 张艳萍 E-mail:64929217@qq.com
作者简介:赵玮(1976-),男,副研究员,主要从事胡麻耐盐品种选育研究。
基金资助:
国家自然科学基金项目（31560347、31660368、31760426）；兰州市人才创新创业项目（2017-RC-97）；甘肃特色作物产业技术体系（胡麻岗位）；甘肃省农业科学院科研条件建设及成果转化项目（2017GAAS22）

Comprehensive Evaluation for Salinity Tolerance of Flax Resources During Germination

ZHAO Wei¹, QI Yan-Ni¹, ZHANG Jian-Ping¹, ZHAO Li¹, WANG Li-Min¹, XIE Ya-Ping¹, WANG Bin¹, ZHANG Yan-Ping²

1. Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070;
2. Institute of Biotechnology, Gansu Academy of Agricultural Science, Lanzhou 730070

Received:2019-05-31 Online:2019-11-05 Published:2019-11-16
Supported by:
This study was supported by National Natural Science Fundation of China(31560347,31660368,31760426);Lanzhou Talent Innovation and Entrepreneurship Project(2017-RC-97);Technical System of Characteristic Crop Industry in Gansu Province(Flax Post);Scientific Research Conditions Construction and Achievement Conversion Project of Gansu Academy of Agricultural Sciences(2017GAAS22)

摘要/Abstract

摘要： 筛选胡麻耐盐品种，对胡麻产业可持续发展应对盐渍胁迫具有重要现实意义。试验选用100 mmol·L^-1的NaCl溶液对28份胡麻资源种子进行胁迫，并测定发芽率、总鲜重、下胚轴鲜重、下胚轴干重、下胚轴长、胚根鲜重、胚根干重和胚根长等相关指标，通过相关性分析、因子和主成分分析、聚类分析和逐步回归分析对11个指标进行综合分析，对28份胡麻资源萌发期耐盐性做出评价，并筛选出胡麻萌发期耐盐性鉴定的关键指标。结果表明：经相关分析，11个指标中多项指标间都是极显著或显著的正相关；因子和主成分分析将11个单项指标转化成3个综合指标，第Ⅰ主成分为下胚轴长势情况，第Ⅱ主成分为胚根长势情况，第Ⅲ主成分为种子萌发情况；聚类分析将28份胡麻资源划分为4大类：耐盐型、中等耐盐型、盐敏感型和强盐敏感型。雁杂10号、轮选1号为强耐盐材料；陇亚4号、R40号、DYMS为较强耐盐材料；逐步回归分析建立了胡麻萌发期耐盐能力预测的数学评价模型，筛选出总鲜重、胚根干重、发芽指数和下胚轴长这4个鉴定指标是胡麻萌发期耐盐能力鉴定的关键指标。以这4个筛选指标计算出的预测值与综合评价计算出的综合Z值之间极显著相关（R²=0.991 8^**），表明鉴定结果具有较高的准确性，可以代替原来11个指标对胡麻萌发期耐盐性进行评价。

关键词: 胡麻, 萌发期, 耐盐性, 综合评价, 指标筛选

Abstract: It has important practical significance for flax to screen salt tolerant varieties to deal with salt stress in the sustainable development In this study, the seed of 28 flax resources were treated with 100 mmol·L^-1 NaCl and the germination rate, total fresh weight, hypocotyl fresh weight, hypocotyl dry weight, hypocotyl length, radicle fresh weight, radicle dry weight, radicle length were measured on the seventh day. Through correlation analysis, factor and principal components analysis, clustering analysis and stepwise regression analysis, the 11 indicators were comprehensively analyzed in order to comprehensively evaluate the salinity tolerance of 28 flax materials during germination, and screen out the key indicators for the salt-tolerance identification of flax materials during germination. The results showed that during the correlation analysis, there were extremely significant or significant and positive relationships among multiple indicators; the factor and principal components analysis turned 11 single indicators into 3 comprehensive indicators. The principal component Ⅰ mainly reflected the growth situation of hypocotyl; The principal component Ⅱ mainly reflected the growth situation of radicle; The principal component Ⅲ mainly reflected the germination situation of seed. The 28 flax materials were divided into 4 categories by clustering analysis:salt tolerant, medium salt tolerant, salt sensitive, and strongly salt sensitive. Among 28 flax materials, Yanza 10 and Lunxuan 1 were strongly salt tolerant; Longya 4、R40 and DYMS were salt tolerant. We built a mathematical evaluation model for predicting the salinity tolerance of flax resources during germination, then screened out 4 key indicators for salt-tolerance identification of flax resources during germination, including total fresh weight, radicle dry weight, germination index and hypocotyl length. The predictive values of 4 key indicators had a significantly correlation with the comprehensive Z values(R²=0.991 8^**), which showed that the results were high accuracy, so the 4 key indicators could evaluate the salinity tolerance of flax resources during germination by taking place of 11 indicators.

Key words: Flax, germination, salinity, comprehensive evaluation, indicators screening

中图分类号:

S563.2

赵玮, 齐燕妮, 张建平, 赵利, 王利民, 谢亚萍, 王斌, 张艳萍. 胡麻资源萌发期耐盐综合性评价[J]. 植物研究, 2019, 39(6): 955-963.

ZHAO Wei, QI Yan-Ni, ZHANG Jian-Ping, ZHAO Li, WANG Li-Min, XIE Ya-Ping, WANG Bin, ZHANG Yan-Ping. Comprehensive Evaluation for Salinity Tolerance of Flax Resources During Germination[J]. Bulletin of Botanical Research, 2019, 39(6): 955-963.

参考文献

1. 赵玮,党占海,李闻娟. 强抗旱胡麻新品种陇亚11号组织培养技术优化研究[J]. 中国沙漠,2012,32(5):1355-1361. Zhao W,Dang Z H,Li W J. Optimization of tissue culture techniques for a drought-resistant Longya 11 variety of flax[J]. Journal of Desert Research,2012,32(5):1355-1361.
2. 赵利,赵玮,李闻娟,等. 不同环境下胡麻脂肪酸含量的遗传分析[J]. 干旱地区农业研究,2018,36(6):48-55. Zhao L,Zhao W,Li W J,et al. Genetic analysis of fatty acid contents in flax(Linum usitatissimum L.) grown under different environmental conditions[J]. Agricultural Research in the Arid Areas,2018,36(6):48-55.
3. 党占海,赵蓉英,王敏,等. 国际视野下胡麻研究的可视化分析[J]. 中国麻业科学,2010,32(6):305-313. Dang Z H,Zhao R Y,Wang M,et al. Visualization analysis on oil flax research-based on the view of internationalization[J]. Plant Fiber Sciences in China,2010,32(6):305-313.
4. 史晓龙,张智猛,戴良香,等. 外源施钙对盐胁迫下花生营养元素吸收与分配的影响[J]. 应用生态学报,2018,29(10):3302-3310. Shi X L,Zhang Z M,Dai L X,et al. Effects of calcium fertilizer application on absorption and distribution of nutrients in peanut under salt stress[J]. Chinese Journal of Applied Ecology,2018,29(10):3302-3310.
5. 崔云玲,王生录,陈炳东. 油葵耐盐性试验研究[J]. 甘肃农业科技,2002(8):35-38. Cui Y L,Wang S L,Chen B D. Trial study on oil sunflower salt tolerance[J]. Gansu Agricultural Science and Technology,2002(8):35-38.
6. 郭文芳,农万廷,李刚强,等. 植物耐盐碱基因工程研究进展[J]. 生物技术通报,2015,31(7):11-17. Guo W F,Nong W T,Li G Q,et al. Research progress of genetic engineering on plant salt tolerance[J]. Biotechnology Bulletin,2015,31(7):11-17.
7. 李惠根,王晓宇,杨文军,等. 两个蓖麻品种萌发期的耐盐性与相关基因响应的差异比较[J]. 分子植物育种,2018,16(22):7473-7480. Li H G,Wang X Y,Yang W J,et al. Comparison of salt tolerance and salt-responsive genes expression in two different varieties of castor(Ricinus communis) at germination stage[J]. Molecular Plant Breeding,2018,16(22):7473-7480.
8. 侯文焕,赵艳红,唐兴富,等. 菜用黄麻种质萌发期耐盐性评价[J]. 植物遗传资源学报,2019,20(2):309-320. Hou W H,Zhao Y H,Tang X F,et al. Assessment for salt tolerance in vegetable jute germplasms at the germination stage[J]. Journal of Plant Genetic Resources,2019,20(2):309-320.
9. 郭媛,邱财生,龙松华,等. 耐盐碱亚麻种质的筛选与综合评价[J]. 中国麻业科学,2015,37(6):285-290. Guo Y,Qiu C S,Long S H,et al. Screening and comprehensive evaluation of salt-alkali tolerant flax germplasm[J]. Plant Fiber Sciences in China,2015,37(6):285-290.
10. 于莹,程莉莉,赵东升,等. 亚麻品种萌发期耐盐性鉴定[J]. 黑龙江农业科学,2015(1):1-5. Yu Y,Cheng L L,Zhao D S,et al. Salt tolerance identification of flax varieties at germination stage[J]. Heilongjiang Agricultural Sciences,2015(1):1-5.
11. 唐启义. DPS数据处理系统:实验设计、统计分析及数据挖掘:2版[M]. 北京:科学出版社,2010:784-789. Tang Q Y. Data processing system:experimental design、statistical analysis and data mining:2nd ed[M]. Beijing:Science Press,2010:784-789.
12. 刘雅辉,王秀萍,左永梅,等. 蒲公英苗期盐胁迫反应及耐盐阈值的确定[J]. 西北农业学报,2017,26(8):1223-1229. Liu Y H,Wang X P,Zuo Y M,et al. Response of dandelion to saline stress and determination of salt-tolerance threshold at seedling stage[J]. Acta Agriculturae Boreali-occidentalis Sinica,2017,26(8):1223-1229.
13. 李源,刘贵波,高洪文,等. 紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J]. 草业学报,2010,19(4):79-86. Li Y,Liu G B,Gao H W,et al. A comprehensive evaluation of salt-tolerance and the physiological response of Medicago sativa at the seedling stage[J]. Acta Prataculturae Sinica,2010,19(4):79-86.
14. Ahmad S,Khan N U I,Iqbal M Z,et al. Salt tolerance of cotton(Gossypium hirsutum L.)[J]. Asian Journal of Plant Sciences,2002,1(6):715-719.
15. 刘宝玉,张文辉,刘新成,等. 沙枣和柠条种子萌发期耐盐性研究[J]. 植物研究,2007,27(6):721-728. Liu B Y,Zhang W H,Liu X C,et al. Salt tolerance of Elaeagnus angustifolia L. and Caragana korshinskii Kom. during germination[J]. Bulletin of Botanical Research,2007,27(6):721-728.
16. 于莹,吴广文,黄文功,等. 2个亚麻品种萌发期耐盐碱性比较研究[J]. 中国麻业科学,2013,35(3):139-143. Yu Y,Wu G W,Huang W G,et al. Comparative study on saline-alkaline tolerance between two flax varieties at germination stage[J]. Plant Fiber Sciences in China,2013,35(3):139-143.
17. 姜珊,张文辉,刘新成. 3种园林树木种子萌发期耐盐性研究[J]. 西北植物学报,2009,29(4):733-741. Jiang S,Zhang W H,Liu X C. Study on salt stress of 3 ornamental trees during germination period[J]. Acta Botanica Boreali-Occidentalia Sinica,2009,29(4):733-741.
18. 张欢欢,马东方,柏新富,等. 玉米幼苗对NaCl胁迫的生理响应和耐受阈值分析[J]. 鲁东大学学报:自然科学版,2013,29(4):324-329. Zhang H H,Ma D F,Bai X F,et al. An analysis on the responsiveness and the tolerant threshold of maize seedlings to NaCl stress[J]. Ludong University Journal:Natural Science Edition,2013,29(4):324-329.
19. Moud A M,Maghsoudi K. Salt stress effects on respiration and growth of germinated seeds of different wheat(Triticum aestivum L.) cultivars[J]. World Journal of Agricultural Sciences,2008,4(3):351-358.
20. Azuma R,Ito N,Nakayama N,et al. Fruits are more sensitive to salinity than leaves and stems in pepper plants(Capsicum annuum L.)[J]. Scientia Horticulturae,2010,125(3):171-178.
21. 徐宁,陈冰嬬,王明海,等. 绿豆品种资源萌发期耐碱性鉴定[J]. 作物学报,2017,43(1):112-121. Xu N,Chen B R,Wang M H,et al. Identification of alkali tolerance of mungbean germplasm resources during germination[J]. Acta Agrpnomica Sinica,2017,43(1):112-121.
22. Azhar F M,Mcneilly T. Variability for salt tolerance in Sorghum bicolor(L.) Moench. under hydroponic conditions[J]. Journal of Agronomy and Crop Science,1987,159(4):269-277.
23. Maiti R K,Maiti L E,Maiti S,et al. Genotypic variability in maize cultivars(Zea mays L.) for resistance to drought and salinity at the seedling stage[J]. Journal of Plant Physiology,1996,148(6):741-744.
24. Kebebew F,Mcneilly T. Variation in response of accessions of minor millets,Pennisetum americanum(L.) Leeke (pearl millet) and Eleusine coracana(L.) Gaertn (finger millet),and Eragrostis tef(Zucc.) Trotter (tef) to salinity in early seedling growth[J]. Plant and Soil,1995,175(2):311-321.
25. 田伯红,王素英,李雅静,等. 谷子地方品种发芽期和苗期对NaCl胁迫的反应和耐盐品种筛选[J]. 作物学报,2008,34(12):2218-2222. Tian B H,Wang S Y,Li Y J,et al. Response to sodium chloride stress at germination and seedling and identification of salinity tolerant genotypes in foxtail millet landraces originated from China[J]. Acta Agronomica Sinica,2008,34(12):2218-2222.

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胡麻资源萌发期耐盐综合性评价

Comprehensive Evaluation for Salinity Tolerance of Flax Resources During Germination

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