不同育性三角梅品种之间的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究

doi:10.7525/j.issn.1673-5102.2018.06.009

植物研究 ›› 2018, Vol. 38 ›› Issue (6): 860-868.doi: 10.7525/j.issn.1673-5102.2018.06.009

不同育性三角梅品种之间的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究

徐夙侠¹, 黄青云¹, 林春松¹, 徐一丁², 李紫阳³, 刘育梅⁴, 黄一锦⁵

1. 福建省亚热带植物研究所福建省亚热带植物生理生化重点实验室, 厦门 361006;
2. 北京林业大学园林学院, 北京 100083;
3. 东北农业大学农学院, 哈尔滨 150030;
4. 厦门华侨亚热带植物引种园厦门市植物引种检疫与植物源产物重点实验室, 厦门 361002;
5. 厦门大学附属第一医院皮肤科, 厦门 361003

收稿日期:2018-03-12 出版日期:2018-11-15 发布日期:2018-11-01
作者简介:XU Su-Xia(1973-),female,major in plont resources.
基金资助:
This study was supported by the National Natural Science Foundation of China(31071814;31372093);Xiamen Science & Technology Project(3502Z20172015);Xiamen Creation Plantform of Science & Technology(3502Z20131004)

Comparison of Reproductive Biology Including Morphology of Reproductive Organs,Viability of Pollens,Flower Visitors,and Flavonols Accumulation Between Fertile and Sterile Cultivars in Bougainvillea

XU Su-Xia¹, HUANG Qing-Yun¹, LIN Chun-Song¹, XU Yi-Ding², LI Zi-Yang³, LIU Yu-Mei⁴, HUANG Yi-Jin⁵

1. Key Laboratory of Fujian Provincial Subtropical Plant Physiology & Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006;
2. School of Landscape Architecture, Beijing Forestry University, Beijing 100083;
3. Department of Agriculture, Northeast Agricultural University, Harbin 150030;
4. Plant Introduction & Quarantine and Plant Product Key Laboratory of Xiamen City, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002;
5. The First Affiliated Hospital of Xiamen University, Xiamen 361003

Received:2018-03-12 Online:2018-11-15 Published:2018-11-01
Supported by:
This study was supported by the National Natural Science Foundation of China(31071814;31372093);Xiamen Science & Technology Project(3502Z20172015);Xiamen Creation Plantform of Science & Technology(3502Z20131004)

摘要/Abstract

摘要： 三角梅高度不育，到目前为止其不育机理仍未理解透彻。本研究通过比较三角梅中可育和不育品种之间的生殖器官特征、花粉的活力、造访昆虫以及花粉中的类黄酮积累差异，为揭示其不育机理提供更多的证据。四个可育和两个不育三角梅品种的花萼管比较结果显示，可育品种的花萼管饱满呈长卵形，而不育品种的花萼管细长管状。可育品种的花粉形态均匀，而不育品种的花粉大小不一。花粉活性结果显示，可育品种的花粉萌发率在11.1%~46.5%，不育品种的花粉在相同的培养条件下几乎不萌发。在可育和不育品种间，共有七种昆虫造访，但昆虫的种类没有表现出明显的差异和偏好。类黄酮积累调查结果显示山奈酚/槲皮素糖苷衍生物是花粉中的主要类黄酮组分。利用花粉荧光染料DPBDA对四个可育和两个不育三角梅品种花器官中的黄酮醇含量比较显示，可育材料中的两种黄酮醇的含量显著高于不育材料，花粉管尖端的荧光信号相比花粉管的其他位置更丰富。我们推测黄酮醇含量差异，特别是槲皮素含量可能对三角梅花粉的活力有一定的积极作用。

关键词: 三角梅, 育性, 花粉, 活力, 黄酮醇

Abstract: Plants in Bougainvillea are characterized with high sterility. In this study, morphology of reproductive organs, viability of pollens, flower visitors, and flavonols accumulation were compared between two sterile and four fertile cultivars. The calyx in four fertile cultivars was oval and bigger than that in other sterile cultivars. Pollens were size-consistent in fertile cultivars. Viability of pollens with 11.1 to 46.5 germination rate in fertile cultivars was much higher than that in the sterile. Seven kinds of insect visitors were found in six cultivars, which displayed no discrimination among various cultivars. The results indicated that kaempferol/and quercetin/glycosylated derivatives were the major flavonols accumulating in pollens using the florescence dye, diphenylboric acid 2-aminoethyl ester (DPBA). Florescence strength in tips of pollen tubes was much robuster than that in other parts. Accumulation of flavonols resulted in sharp difference between fertile cultivars and sterile cultivars in the study, which suggested that flavonols especially quercetin might play positive roles in fertility of plants in Bougainvillea.

Key words: Bougainvillea, fertility, pollen, viability, flavonol

中图分类号:

Q949.745.3

徐夙侠, 黄青云, 林春松, 徐一丁, 李紫阳, 刘育梅, 黄一锦. 不同育性三角梅品种之间的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究[J]. 植物研究, 2018, 38(6): 860-868.

XU Su-Xia, HUANG Qing-Yun, LIN Chun-Song, XU Yi-Ding, LI Zi-Yang, LIU Yu-Mei, HUANG Yi-Jin. Comparison of Reproductive Biology Including Morphology of Reproductive Organs,Viability of Pollens,Flower Visitors,and Flavonols Accumulation Between Fertile and Sterile Cultivars in Bougainvillea[J]. Bulletin of Botanical Research, 2018, 38(6): 860-868.

参考文献

1. Mabberley D J. The Plant Book[M]. Cambridge:Cambridge Univ Press,1997:1-706.
2. Balasaraswathi R,Sadasivam S,Ward M,et al. An antiviral protein from Bougainvillea spectabilis roots;purification and characterisation[J]. Phytochemistry,1998,47(8):1561-1565.
3. Schlein Y,Jacobson R L,Muller G C. Sand fly feeding on noxious plants:a potential method for the control of Leishmaniasis[J]. Am J Trop Med Hyg,2001,65(4):300-303.
4. Joseph O,Adebay O,Adesokan A A,et al. Effect of ethanolic extract of Bougainvillea spectabilis leaves on hematological and serum lipid variables in rats[J]. Biochemistry,2005,17(1):45-50.
5. Ali M S,Ibrahin S A,Ahmed F,et al. Color versus bioactivity in the flowers of Bougainvillea spectabilis (Nyctaginaceae)[J]. Nat Prod Res,2005,19(1):1-5.
6. Sharma S C,Srivastava R,Roy R K. Role of Bougainvilleas in mitigation of environmental pollution[J]. Environ Sci Eng,2005,47(2):131-134.
7. Narayanan C R,Joshi D D,Mujumdar A M,et al. Pinitol-a newanti-diabetic compound from the leaves of Bougainvillea spectabilis[J]. Curr Sci,1987,56:139-141.
8. Sarah H B,Jones R B,Bailey C J. Insulin-like effect of pinitol[J]. Brit J Pharm,2000,30:1944-1948.
9. Campbell W W,Haub M D,Fluckey J D,et al. Pinitol supplementation does not affect insulin-mediated glucose metabolism and muscle insulin receptor content and phosphorylation in older humans[J]. Nutr,2004,4:2998-3003.
10. Zadoo S,Khoshoo T N. Cytogenetical studies in Bougainvillea. Ⅰ. A case of interchange heterozygosity[J]. Genetica,1968,39:353-359.
11. Khoshoo T N,Zadoo S. New perspectives in Bougainvillea breeding[J]. Hered,1969,60:357-360.
12. Xu S X,Huang Q Y,Shu Q Y,et al. Reproductive Organography of Bougainvillea spectabilis Willd[J]. Sci Hortic,2009,120(3):399-405.
13. López H A,Galetto L. Flower Structure and Reproductive Biology of Bougainvillea stipitata(Nyctaginaceae)[J]. Plant Biology,2002,4(04):508-514.
14. Coe E H,McCormick S M,Modena S A. White pollen in maize[J]. Hered,1981,72:318-320.
15. Mo Y Y,Nagel C,Taylor L P. Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen[J]. Proc Natl Acad Sci USA,1992,89:7213-7217.
16. Taylor L P,Grotewold E. Flavonoids as developmental regulators[J]. Curr Opin Plant Biol,2005,8:317-323.
17. Lewis D R,Ramirez M V,Miller N D,et al. Auxin and ethylene induce flavonol accumulation through distinct transcriptional networks[J]. Plant Physiol,2011,156:144-164.
18. Sheahan J J,Rechnitz G A. Differential visualization of transparent testa mutants in Arabidopsis thaliana[J]. Anal Chem,1993,65:961-963.
19. Peer W A,Brown D E,Tague B W,et al. Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis[J]. Plant Physiol,2001,126:536-548.
20. Warkins J M,hechler P J,Muday G K. Ethylene-induced flavonol accumulation in guard cells suppresses reactive oxygen cultivars and moderates stomatal aperture[J]. Plant Physiol,2014,164:1707-1717.
21. Hertog M G L,Hollman P C H,Venema D P. Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits[J]. Agric Food Chem,1992a,40:1591-1598.
22. Yang R Z,Wei X L,Gao F F,et al. Simultaneous analysis of anthocyanins and flavonols in petals of lotus(Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry[J]. Chromatography A,2009,1216:106-112.
23. Mahajan M,Ahuja P S,Yadav S K. Post-Transcriptional Silencing of Flavonol Synthase mRNA in Tobacco Leads to Fruits with Arrested Seed Set[J]. PLoS ONE,2011,6(12):e28315.
24. Pollak P E,Vogt T,Mo Y,et al. Chalcone synthase and flavonol accumulation in stigmas and anthers of Petunia hybrida[J]. Plant Physiol,1993,102:925-932.
25. Ylstra B,Touraev A,Benito Moreno R M,et al. Flavonols stimulate development,germination and tube growth of tobacco pollen[J]. Plant Physiol,1992,100:902-907.

[1]	王丹, 张中帅, 曾庆银, 韩学敏. 滇西北冷杉属植物结实特性及种子特征研究[J]. 植物研究, 2023, 43(5): 647-656.
[2]	张淑钧, 杨欣欣, 罗建. 西藏色季拉山区风毛菊属植物花粉形态特征及其分类学意义[J]. 植物研究, 2023, 43(5): 741-755.
[3]	杨庆华, 葛斌杰, 张大生. 唇形科兰香草蓝色花粉形成观察与成分分析[J]. 植物研究, 2023, 43(5): 794-800.
[4]	张亚利, 杨耐英, 宋垚, 李湘鹏, 郭卫珍, 蔡友铭. 20种山茶属连蕊茶组和毛蕊茶组植物的花粉形态特征研究[J]. 植物研究, 2023, 43(3): 470-480.
[5]	杨建伟, 李宗艳, 冯尧, 任书娴, 胡梦露, 叶松菩. 束花石斛的繁育生物学特性[J]. 植物研究, 2023, 43(1): 150-160.
[6]	汪颖, 张玉洁, 焦子惠, 李雪丽, 王凯, 达晓伟, 孙坤, 张辉. 中国‘苦水’玫瑰杂交育性的初步研究[J]. 植物研究, 2022, 42(3): 403-411.
[7]	陆敏, 李西林, 陆雄. 花粉块内花粉粒形态观察的扫描电镜样品制备方法[J]. 植物研究, 2021, 41(5): 836-840.
[8]	李洪池, 吴天彧, 弓莉, 罗建. 西藏地区13种虾脊兰植物花部表型及花粉形态特征研究[J]. 植物研究, 2021, 41(4): 547-556.
[9]	吕东林, 李腾, 郭译文, 姜静, 黄海娇. 转基因白桦杂交子代种子活力及外源基因遗传规律分析[J]. 植物研究, 2021, 41(4): 564-572.
[10]	王艳萍, 李璐, 杨晨璇, 罗艳, 李青青. 14种石斛属（兰科）植物的花粉团形态及分类学意义[J]. 植物研究, 2021, 41(1): 12-25.
[11]	杨晨, 陈志祥, 姚雪莹, 王河山, 王奇志. 中国15种变豆菜属植物的花粉形态及系统学分析[J]. 植物研究, 2020, 40(6): 805-812.
[12]	赵历强, 单春苗, 张声祥, 施圆圆, 马克龙, 吴家文. 基于转录组测序的细风轮花青素合成途径及关键酶基因分析[J]. 植物研究, 2020, 40(6): 886-896.
[13]	梁玲, 江洁蓓, 张腾驹, 吕佳瑶, 陈小红. 不同色彩珙桐苞片与叶片的生理特性研究[J]. 植物研究, 2020, 40(4): 505-513.
[14]	李璐, 张锦, 李楚然, 王艳萍, 谭庆琴, 罗艳. 兰科新种巾唇兰的花粉团发育及分类学意义[J]. 植物研究, 2020, 40(2): 161-171.
[15]	林琳, 高宇婷, 程福山, 辛本花, 王贵春, 夏富才, 穆怀志. 赛黑桦不同半同胞家系种子活力比较[J]. 植物研究, 2020, 40(1): 125-132.

不同育性三角梅品种之间的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究

Comparison of Reproductive Biology Including Morphology of Reproductive Organs,Viability of Pollens,Flower Visitors,and Flavonols Accumulation Between Fertile and Sterile Cultivars in Bougainvillea

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价