基于cas9/gRNA创制毛果杨PtrFLA31/34突变体

doi:10.7525/j.issn.1673-5102.2018.05.014

植物研究 ›› 2018, Vol. 38 ›› Issue (5): 741-747.doi: 10.7525/j.issn.1673-5102.2018.05.014

基于cas9/gRNA创制毛果杨PtrFLA31/34突变体

位珍, 程玉祥, 夏德安

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2017-12-27 出版日期:2018-09-15 发布日期:2018-08-31
通讯作者: 夏德安 E-mail:xiadean@126.com
作者简介:位珍(1991-),女,硕士,主要从事林木次生生长研究。
基金资助:
国家自然科学基金（31570580）

Cas9/gRNA-mediated Targeted PtrFLA31/34 Gene Mutation in Populus

WEI Zhen, CHENG Yu-Xiang, XIA De-An

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2017-12-27 Online:2018-09-15 Published:2018-08-31
Supported by:
National 863 project(31570580)

摘要/Abstract

摘要： 束状阿拉伯半乳聚糖蛋白（FLA）对植物生长发育都起着重要作用。基于生物信息学分析，我们识别毛果杨FLA家族有46个成员；进化分析显示毛果杨FLA家族分为A、B、C、D 4个亚族，且PtrFLA31和PtrFLA34处在进化树的一个小分支。半定量RT-PCR分析表明，PtrFLA31和PtrFLA34在毛果杨木质组织特异性、高水平表达。为了鉴定PtrFLA31和PtrFLA34在木材形成上的功能，我们用Cas9/gRNA技术敲除PtrFLA31/34，获得3株毛果杨fla31/34敲除双基因突变体材料。

关键词: 毛果杨, PtrFLA, 基因突变体, Cas9/gRNA

Abstract: Fasciclin-like arabinogalactan proteins(FLAs) play important roles in growth and development of plants. Based on bioinformatic analysis, we identified 46 FLAs in Populus trichocarpa. By phylogenetic analysis, Populus FLA family was classified into four Sub-groups(A-D) and PtrFLA31 and PtrFLA34 were in same small branch of the tree. By semi-quantitative RT-PCR analysis, PtrFLA31 and PtrFLA34 were specifically expressed in xylem on the high transcriptional level. In order to study their functions in wood formation, we knocked out PtrFLA31/34 double genes via Cas9/gRNA, and obtained three fla31/34 double mutants.

Key words: Populus trichocarpa, PtrFLA, mutant, Cas9/gRNA

中图分类号:

S792.11

位珍, 程玉祥, 夏德安. 基于cas9/gRNA创制毛果杨PtrFLA31/34突变体[J]. 植物研究, 2018, 38(5): 741-747.

WEI Zhen, CHENG Yu-Xiang, XIA De-An. Cas9/gRNA-mediated Targeted PtrFLA31/34 Gene Mutation in Populus[J]. Bulletin of Botanical Research, 2018, 38(5): 741-747.

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基于cas9/gRNA创制毛果杨PtrFLA31/34突变体

Cas9/gRNA-mediated Targeted PtrFLA31/34 Gene Mutation in Populus

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