根据广义形态性状和nrDNA ITS序列试论长颖固沙草的归并

doi:10.7525/j.issn.1673-5102.2017.02.003

植物研究 ›› 2017, Vol. 37 ›› Issue (2): 174-180.doi: 10.7525/j.issn.1673-5102.2017.02.003

根据广义形态性状和nrDNA ITS序列试论长颖固沙草的归并

刘玉萍, 吕婷, 周勇辉, 张晓宇, 苏旭

青海师范大学生命与地理科学学院, 青藏高原药用动植物资源重点实验室, 青海省自然地理与环境过程重点实验室, 青藏高原环境与资源教育部重点实验室, 西宁 810008

收稿日期:2016-11-07 出版日期:2017-03-15 发布日期:2017-03-11
通讯作者: 苏旭,E-mail:xusu8527972@126.com E-mail:xusu8527972@126.com
作者简介:刘玉萍(1980-),女,博士,副教授,主要从事系统与进化植物学研究。
基金资助:
国家自然科学基金项目（31260052）；青海省自然科学基金项目（2016-ZJ-937Q，2014-ZJ-947Q）；青藏高原药用动植物资源重点实验室项目（2015-Z-Y06）；青海师范大学中青年科研基金项目（2015-19）

Reduction on Orinus longiglumis with Generalized Morphological Characters and nrDNA ITS Sequence

LIU Yu-Ping, LÜ Ting, ZHOU Yong-Hui, ZHANG Xiao-Yu, SU Xu

Key Laboratory of Medicinal Plant and Animal Resources in the Qinghai-Tibetan Plateau, Key Laboratory of Physical Geography and Environmental Process in Qinghai Province, Key Laboratory of Education Ministry of Environments and Resources in the Qinghai-Tibetan Plateau, School of Geography and Life Science, Qinghai Normal University, Xi'ning 810008

Received:2016-11-07 Online:2017-03-15 Published:2017-03-11
Supported by:
Under the auspices of the National Natural Science Foundation of China(No.31260052);The National Natural Science Foundation of Qinghai Province(2016-ZJ-937Q,2014-ZJ-947Q);The Key Laboratory of Medicinal Plant and Animal Resourcesofthe Qinghai-Tibetan Plateau(2015-Z-Y06);The Young and Middle-Aged Research Foundation of Qinghai Normal University(2015-19)

摘要/Abstract

摘要： 固沙草属是禾本科中一个具有重要经济价值的高山特有属，共记载6个物种，它们均具有较强的抗逆特性，是农业良种繁育、畜牧业牧草改良利用的重要资源；并且，固沙草属物种形态差异不显著，种间界限较模糊。本研究通过广义形态性状的观测、统计和分析，同时结合核基因组ITS片段的研究，系统探讨了长颖固沙草、固沙草和鸡爪草3个近缘物种的主要形态性状和分子序列的差异，旨在评判长颖固沙草的系统分类归属。研究表明，在广义形态性状上长颖固沙草与固沙草差异甚小，难以进行区分，但与鸡爪草差异明显，是典型的种间关系；从基于ITS序列构建的系统发育树看，3个物种中只形成两个比较明显的遗传分支，一个分支是由长颖固沙草和与固沙草所测个体构成，另一分支仅由鸡爪草所测个体组成，并且分支的靴带支持率高达100%。故本研究认为，长颖固沙草与固沙草应是同一个物种，长颖固沙草应作为固沙草的异名处理。

关键词: 禾本科, 固沙草属, 长颖固沙草, 形态性状, ITS序列

Abstract: Orinus is an alpine genus with important economic values in the grass family(Poaceae). There are six species in it and all of them have stronger adverse-resistant characteristics, and they are the important resources for improving and applying agricultural breeding and animal husbandry. Meanwhile, the discriminative characters are obscure among these species. We compared and analyzed the main morphological characters and molecular sequence differences among nearest related Orinus longiglumis, O.thoroldii and O.anomala by generalized morphological observation and nuclear ITS region research. There is very slight disparity between O.longiglumis and O.thoroldii in generalized morphological characters, the classification of the two species is very difficult, the differences between O.longiglumis and O.anomala are distinct, and the two taxa are two different species. We further used the ITS sequence variation to tentatively delimit the previously acknowledged species and found that there is no distinct boundary between species and only two distinct lineages were recognized for all three species. A branch is composed of O.longiglumis and O.thoroldii, while another is only made up of O.anomala. The bootstrap support rates of two branches reach as high as 100%. Therefore, O.longiglumis and O.thoroldii should belong to the same species, and O.longiglumis can be reduced to synonymy of O.thoroldii.

Key words: Poaceae, Orinus, Orinus longiglumis, morphological character, ITS sequence

中图分类号:

Q949.71

刘玉萍, 吕婷, 周勇辉, 张晓宇, 苏旭. 根据广义形态性状和nrDNA ITS序列试论长颖固沙草的归并[J]. 植物研究, 2017, 37(2): 174-180.

LIU Yu-Ping, LÜ Ting, ZHOU Yong-Hui, ZHANG Xiao-Yu, SU Xu. Reduction on Orinus longiglumis with Generalized Morphological Characters and nrDNA ITS Sequence[J]. Bulletin of Botanical Research, 2017, 37(2): 174-180.

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根据广义形态性状和nrDNA ITS序列试论长颖固沙草的归并

Reduction on Orinus longiglumis with Generalized Morphological Characters and nrDNA ITS Sequence

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