基于RNA-seq对南蛇藤MADS-box转录因子序列及表达分析

doi:10.7525/j.issn.1673-5102.2017.01.010

植物研究 ›› 2017, Vol. 37 ›› Issue (1): 69-77.doi: 10.7525/j.issn.1673-5102.2017.01.010

基于RNA-seq对南蛇藤MADS-box转录因子序列及表达分析

祖奎玲, 董树斌, 李建霞, 赵芸玉, 赵良成

北京林业大学自然保护区学院, 北京 100083

收稿日期:2016-07-06 出版日期:2017-01-15 发布日期:2017-03-06
通讯作者: 赵良成,E-mail:lczhao@bjfu.edu.cn E-mail:lczhao@bjfu.edu.cn
作者简介:祖奎玲(1991-),女,硕士研究生,主要研究方向是植物发育分子生物学。
基金资助:
国家自然科学基金（31370213）；北京市自然科学基金（5133036）

Sequence and Expression Analysis of MADS-box Genes in Celastrus orbiculatus Thunb. Based on RNA-seq Data

ZU Kui-Ling, DONG Shu-Bin, LI Jian-Xia, ZHAO Yun-Yu, ZHAO Liang-Cheng

School of Nature Reservation, Beijing Forestry University, Beijing 100083

Received:2016-07-06 Online:2017-01-15 Published:2017-03-06
Supported by:
This work was supported by funding from the National Natural Science Foundation of China(31370213);Beijing Natural Science Foundation(5133036)

摘要/Abstract

摘要： 为了分析南蛇藤MADS-box转录因子对其雌花发育和果实形成的调控信息，本文基于南蛇藤的转录组数据，获得15个具有全长开放阅读框的MADS-box转录因子（ColMADS），并利用生物信息学方法对其性质和结构特性进行了分析。结果表明，基因comp37814_g1和comp41380_g2属于M-type家族，不含有K盒结构，其他均为MIKC家族；除了comp37713_g1之外，其余均属于不稳定的亲水性蛋白；二级结构均含有α-螺旋、扩展链结构、β-转角和无规则卷曲，其中α-螺旋所占比例最高；序列主要包含5种保守基序，基序1和基序2分别含50个氨基酸且属于该基因家族的保守基序，仅基序1含有MADS盒。系统进化分析结果显示南蛇藤ColMADS转录因子被分为10个进化支，分属于不同类型的亚家族及不同的组。另外，利用荧光定量PCR检测方法揭示了这些基因在南蛇藤盛花、落花和幼果不同发育时期的表达情况。结果表明，2个基因在盛花期相对表达值最高，9个基因在落花期相对表达值最高，在幼果形成过程中有4个基因显著上调表达。

关键词: 南蛇藤, MADS-box基因, 雌花, 序列及表达分析

Abstract: For the regulatory information of MADS-box genes on the flower and fruit development in Celastrus orbiculatus Thunb, 15 MADS-box genes(ColMADS) with full ORF(Open Reading Frame)were identified from transcriptome generated from female flower and fruit. The features of the proteins were analyzed by the bioinformatics methods. The comp37814_g1and comp41380_g2 belonged to M-type family, while other 13 genes were the members of MIKC. All were unstable hydrophilic proteins except for comp37814_g1. Alpha helix, beta turn, extended strand and random coil were found from the secondary structure of ColMADS protein, and alpha helix was major part. Five motifs were identified. The Motif 1 and Motif 2, both consisting of 50 amino acids, were the conserved structure of MADS-box family and only Motif 1 contained MADS domain. By phylogenetic analysis, 15 ColMADS genes were divided into 10 clades, belonging to different subfamilies and groups. In addition, the expression patterns of the genes among different development stages were analyzed using real-time RT-PCR. Two genes were highly expressed at flower blooming stage and nine genes were obviously expressed at flower senescence stage. There are four genes upregulated during the young fruit development.

Key words: Celastrus orbiculatus Thunb., MADS-box genes, female flower, sequence and expression analysis

中图分类号:

Q949.754.7

祖奎玲, 董树斌, 李建霞, 赵芸玉, 赵良成. 基于RNA-seq对南蛇藤MADS-box转录因子序列及表达分析[J]. 植物研究, 2017, 37(1): 69-77.

ZU Kui-Ling, DONG Shu-Bin, LI Jian-Xia, ZHAO Yun-Yu, ZHAO Liang-Cheng. Sequence and Expression Analysis of MADS-box Genes in Celastrus orbiculatus Thunb. Based on RNA-seq Data[J]. Bulletin of Botanical Research, 2017, 37(1): 69-77.

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基于RNA-seq对南蛇藤MADS-box转录因子序列及表达分析

Sequence and Expression Analysis of MADS-box Genes in Celastrus orbiculatus Thunb. Based on RNA-seq Data

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