刚毛柽柳ThDREB基因在酵母中的表达及抗逆能力分析

doi:10.7525/j.issn.1673-5102.2017.01.009

植物研究 ›› 2017, Vol. 37 ›› Issue (1): 63-68.doi: 10.7525/j.issn.1673-5102.2017.01.009

刚毛柽柳ThDREB基因在酵母中的表达及抗逆能力分析

冯德明¹, 温佩颖¹, 赵畅¹, 杨远彪², 杨桂燕¹, 于丽丽¹, 高彩球¹

1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040;
2. 东北林业大学帽儿山教学区, 尚志 150040

收稿日期:2016-06-21 出版日期:2017-01-15 发布日期:2017-03-06
通讯作者: 高彩球,E-mail:gaocaiqiu@nefu.edu.cn E-mail:gaocaiqiu@nefu.edu.cn
作者简介:冯德明(1995-),女,本科,主要从事林木遗传育种研究。
基金资助:
大学生创新实验训练项目（201510225071）；黑龙江省教育厅科学技术研究项目（12523017）

Expression and Stress Tolerance Charaterization of a ThDREB Gene from Tamarix hispida in Yeast

FENG De-Ming¹, WEN Pei-Ying¹, ZHAO Chang¹, YANG Yuan-Biao², YANG Gui-Yan¹, YU Li-Li¹, GAO Cai-Qiu¹

1. Northeast Forestry University, State Key Laboratory of Tree Genetics and Breeding, Harbin 150040;
2. The Maoershan Teaching Area of Northeast Forestry University, Shangzhi 150040

Received:2016-06-21 Online:2017-01-15 Published:2017-03-06
Supported by:
National-level College Students' Innovative Entrepreneurial Training Plan Program(20151022507) and the Scientific Research Fund of Heilongjiang Provincial Education Department(No.12523017).

摘要/Abstract

摘要： DREB蛋白能特异地与DRE（dehydration responsive element）顺式作用元件结合，从而调控下游多个与逆境相关基因的表达，在植物对多种逆境胁迫的应答反应中起重要调节作用。为了研究刚毛柽柳ThDREB基因是否具有抗逆功能，将ThDREB基因插入到酵母表达载体pYES2中构建成重组载体，转入酿酒酵母（Saccharomyces cerevisiae）中获得重组型酵母。分别比较转ThDREB基因酵母和转空载体对照酵母在山梨醇、H₂O₂、CdCl₂、NaCl、Na₂CO₃、MgCl₂、-20℃胁迫处理之后的存活能力。结果显示，ThDREB基因能有效提高转基因酵母的抗干旱、盐、碱、氧化、重金属及低温胁迫的能力，表明ThDREB基因可能参与了柽柳多种抗逆调控过程。

关键词: 刚毛柽柳, DREB转录因子, 酵母表达, 非生物逆境抗性

Abstract: DREB protein could specifically bind to DRE(dehydration responsive element) cis-acting elements, and regulate the expression of downstream stress related gene. It plays an important role in the regulation responses of plants to abiotic stress. To explore the abiotic stress tolerance role of DREB(ThDREB) from Tamarix hispida, the recombinant plasmid(named as pYES2-ThDREB) was constructed by inserting the ThDREB gene into the yeast expression vector pYES2. The plasmid pYES2-ThDREB was transformed into the yeast Saccharomyces cerevisiae, and the yeast transformed with empty pYES2 was used as the control. The pYES2-ThDREB and pYES2 recombinant yeast were treated with sorbitol, H₂O₂, CdCl₂, NaCl, Na₂CO₃, MgCl₂, and -20℃, respectively. The pYES2-ThDREB recombinant yeast was tolerant to all above abiotic treatments, suggesting that ThDREB maybe an important stress-regulating genes in T.hispida.

Key words: Tamarix hispida, DREB, yeast expression, abiotic stress tolerance

中图分类号:

S793.5

冯德明, 温佩颖, 赵畅, 杨远彪, 杨桂燕, 于丽丽, 高彩球. 刚毛柽柳ThDREB基因在酵母中的表达及抗逆能力分析[J]. 植物研究, 2017, 37(1): 63-68.

FENG De-Ming, WEN Pei-Ying, ZHAO Chang, YANG Yuan-Biao, YANG Gui-Yan, YU Li-Li, GAO Cai-Qiu. Expression and Stress Tolerance Charaterization of a ThDREB Gene from Tamarix hispida in Yeast[J]. Bulletin of Botanical Research, 2017, 37(1): 63-68.

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刚毛柽柳ThDREB基因在酵母中的表达及抗逆能力分析

Expression and Stress Tolerance Charaterization of a ThDREB Gene from Tamarix hispida in Yeast

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