关键词: 萝卜, SRAP, AFLP, 品种指纹图谱

Abstract:

Two molecular marker systems, SRAP and AFLP, were employed to fingerprint the radish cultivars. The SRAP-PCR system was optimized with the concentration of primer at 0.3 μmol·L^-1，dNTPs at 0.2 mmol·L^-1 and Mg²⁺ at 3.0 mmol·L^-1. Similar patterns were obtained from 12 gradient annealing temperature for 50°C with primer em2-me2. The fingerprinting and genetic diversity of seven radish cultivars was analyzed using the optimized SRAP-PCR and AFLP marker system. All cultivars can be distinguished clearly with SRAP primer combinations and 14 AFLP primer combinations. Total of 155 bands were observed with 11 SRAP primer combinations, of which 84 were polymorphic bands. Cluster analysis and relatively genetic distance indicated that all cultivars were clustered into four groups. There was the closest relationship between ‘CB-03-2’ and ‘SHCB-02-1’ with the genetic distance (GD) of 0.054 9 and a distant relationship between ‘Qihongdalian’ and ‘Heiseng’. The cluster analysis of AFLP data showed that all cultivars clustered into three groups with the closest relationship between ‘CB-03-2’ and ‘SHCB-02-1’. The cluster analysis of combination of SRAP and AFLP marker data indicated that ‘CB-03-2’ and ‘SHCB-02-1’ had the closest relationship (GD=0.047 6) and all cultivars clustered into three clusters.

Key words: radish, SRAP, AFLP, cultivar fingerprinting