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植物研究 ›› 2003, Vol. 23 ›› Issue (2): 224-229.

• 论文 • 上一篇    下一篇

红皮云杉群体遗传多样的研究

张含国1, 孙立夫2, 韩继凤3, 丰宝林4   

  1. 1. 黑龙江省林业科学研究所, 哈尔滨 150040;
    2. 东北林业大学, 哈尔滨 150040;
    3. 黑龙江省森工总局信息中心, 哈尔滨 150001;
    4. 铁力林业局, 伊春 152500
  • 收稿日期:2002-08-12 出版日期:2003-06-15 发布日期:2016-06-14
  • 作者简介:张含国(1962-),男,研究员,主要从事遗传育种研究。
  • 基金资助:
    黑龙江省科委重点课题及国家科技攻关项目资助(2002BA515B0402)

STUDY ON THE GENETIC DIVERSITY OF PICEA KORAIENSIS

ZHANG Han-Guo1, SUN Li-Fu2, HAN Ji-Feng3, FENG Bao-Lin4   

  1. 1. Heilongjiang Forestry research institute. Harbin 150040;
    2. Northeast Forestry Univeristy, Harbin 150040;
    3. The information center of Heilongjiang Forest industry Bureau, Harbin 150001;
    4. Tieli Forestry Bureau, Yichun 152500
  • Received:2002-08-12 Online:2003-06-15 Published:2016-06-14

摘要: 在红皮云杉(Picea korainesis)分布区内,选取了有代表性的长白山及其支脉张广才岭、老爷岭、完达山,小兴安岭,大兴安岭,大兴安岭向内蒙古高原过渡地带以及锡霍特山脉的12个群体,运用群体遗传学、生理遗传学、森林遗传学以及林木育种的理论和方法,利用主成分和分层聚类分析等多种统计分析方法,从表型和分子水平等多层次较为系统地研究了红皮云杉群体内、群体间的变异,同时探讨了各种亲代、后代特征以及等位酶与生态环境因子之间的关系。,首次从亲代生物系统学特征,后代苗木形态、生长、生物量和矿物质元素等对红皮云杉群体遗传变异进行了研究,结果表明,红皮云杉具有丰富的遗传变异,群体内变异大于群体间变异;红皮云杉12个群体11个酶系统21个位点中约有27.2%的基因位点是多态的,群体间的变异量只占总变异量的15.2%,84.8%的变异存在于群体内。红皮云杉群体等位酶多态位点的比率在云杉属中处于较低的水平,群体间的分化与云杉其它树种相比处于较高的水平。等位酶与形态等具有相似的变异趋势。根据红皮云杉气候、亲代及后代特征群体区划结果,我们认为红皮云杉种子区划为:长白山种子区(Ⅰ);老爷岭、张广才岭、完达山种子区(Ⅱ);小兴安岭种子区(Ⅲ);大兴安岭北部种子区(Ⅳ);大兴安岭西南部种子区(Ⅴ);锡霍特山脉种子区(Ⅵ)。

关键词: 红皮云杉, 群体, 遗传变异, 种子区划, 遗传改良

Abstract: Twelve populations of Picea koraiensis are collected typically within its distribution from Changbai Mountains, its branches Zhangguanchai Mount, Laoyie Mount and Wanda Mount, to Xiaox-ing'an Mountains and Dasing'an Mountains, with interspersed areas of internai Mogul grassland and Sikshote Mountains. The theories and methods of genetics are applied which include Population Genetics, Ecological Genetics, Physiological Genetics, Molecular Genetics, Forest Genetics and Forest-ry Breeding as well as Statistics such as PCA and Cluster. The variations within population and among populations are systematically studied from phenotype to molecular Ievel and the relationship among the characters of various parents and their progenies, feature in allozyme and their relative ecological environment factors are analyzed. The results indicate as follows:it is first in parent bio-system characters, the morphology, growth, bio-mass and mineral elements of progeny seedlings that the genetic variation of Picea koraiensis is conducted. There are abundant variations in it and its genetic variation within a population is larger than among populations. Among 21 gene loci in 11 enzymes testing, poly-morphic loci makes up about 27.2 percent of total. The variation within population is 15.2 percent and among populations is 84.2 percent. In contrast with other species in Picea spp., the ratio of major allozyme loci within population of Picea koraiensis belongs to lower level, while that among populations reaches to higher Ievel. The genetic variations in allozyme, comparing with morphology, show a similar tendency. According to the characters of parent and progeny of Picea koraiensis and their relative climatic factors, the area of seed allocation are divided into six sections; Ⅰ Changbai Mountains area, Ⅱ Laoyie Mount-Wanda Mount area, Ⅲ Xiaoxing'an Mountains area, Ⅳ North part of Daxing' an Mountains, V Southwest part of Daxing' an Mountains, Ⅵ Sikshote area.

Key words: Picea koraiensis, population, genetic variation, seed divisition, genetic improvement