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植物研究 ›› 2002, Vol. 22 ›› Issue (1): 56-60.

• 论文 • 上一篇    下一篇

香椿试管苗的生根与移栽

张小红, 康冰, 陈彦生, 曹建军, 曹宁   

  1. 西北农林科技大学生命科学学院, 杨凌 712100
  • 收稿日期:2001-10-09 出版日期:2002-03-15 发布日期:2016-06-14
  • 作者简介:张小红(1968-),女,本科学士,主要从事植物组织培养及经济植物快繁技术的研究。

ROOTING AND TRANSPLANTING OF TISSUE CULTURED SHOOTS OF TOONA SINENSIS

ZHANG Xiao-hong, KANG Bing, CHEN Yan-sheng, CAO Jian-jun, CAO Ning   

  1. College of Life Sciences, Northwest Science and Technology University of Agriculture and Forestry, Yang ling 712100
  • Received:2001-10-09 Online:2002-03-15 Published:2016-06-14

摘要: 通过基内诱导生根、基外诱导生根及生根苗移栽等试验,研究了香椿试管苗的生根与移栽。结果表明,基内诱导生根的主要影响因子是生长素IBA,其次是培养基中无机盐浓度,适宜的生根配方是在不含无机盐的培养基中附加1.0mg/L IAB。用NAA、IAB短时间处理无根苗基部,可使部分苗生根,但生根率偏低。试管苗移栽的适宜时期为3月至7月初,以蛭石、河沙为基质效果较好。移载成活的关键是充分炼苗及良好的基质和适宜的时期,注意防虫并保温遮荫。

关键词: 香椿, 试管苗, 生根, 移栽

Abstract: It was studies that the mains factor in fluencing rooting and transplanting of tissue cultured shoots of Toona sinensis. The results were summarized as follows:the main factor influencing rooting of tissue cultured shoots of Toona sinensis is IBA, and the contents of macronutrients in MS medium come second. The proper rooting medium is single IBA 1.0mg/L in MS medium without macronu-tients. The rooting rates was lowly with high concentration of NAA or IBA in a short time. The proper transplanting period is from March to early July every year. Vermiculites or sands is a good transplanting medium. The key to ensure successful transplantation is exercising plants effectively, proper transplanting medium and time. At the same time it is also important that preventing insects, shading and keeping air humidity.

Key words: Toona sinensis, tissue cultured shoots, rooting, transplantation